Djaker N, Brustlein S, Rohman G, Huot S, de la Chapelle M Lamy, Migonney V
Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, CNRS (UMR 7244), 74 rue Marcel Cachin, 93017, Bobigny, France.
Institut Fresnel, MOSAIC, CNRS, Aix-Marseille Université, Ecole Centrale Marseille, Domaine Universitaire St Jérôme, France.
Biomed Opt Express. 2013 Dec 10;5(1):149-57. doi: 10.1364/BOE.5.000149.
Tissue Engineering is a new emerging field that offers many possibilities to produce three-dimensional and functional tissues like ligaments or scaffolds. The biocompatibility of these materials is crucial in tissue engineering, since they should be integrated in situ and should induce a good cell adhesion and proliferation. One of the most promising materials used for tissue engineering are polyesters such as Poly-ε-caprolactone (PCL), which is used in this work. In our case, the bio-integration is reached by grafting a bioactive polymer (pNaSS) on a PCL surface. Using nonlinear microscopy, PCL structure is visualized by SHG and proteins and cells by two-photon excitation autofluorescence generation. A comparative study between grafted and nongrafted polymer films is provided. We demonstrate that the polymer grafting improves the protein adsorption by a factor of 75% and increase the cell spreading onto the polymer surface. Since the spreading is directly related to cell adhesion and proliferation, we demonstrate that the pNaSS grafting promotes PCL biocompatibility.
组织工程是一个新兴领域，它为制造三维功能性组织（如韧带或支架）提供了诸多可能性。这些材料的生物相容性在组织工程中至关重要，因为它们应在原位整合，并应诱导良好的细胞黏附和增殖。用于组织工程的最有前景的材料之一是聚酯，如聚-ε-己内酯（PCL），本研究即使用了这种材料。在我们的研究中，通过在PCL表面接枝生物活性聚合物（pNaSS）实现生物整合。利用非线性显微镜，通过二次谐波产生（SHG）观察PCL结构，通过双光子激发自发荧光产生观察蛋白质和细胞。提供了接枝和未接枝聚合物薄膜之间的对比研究。我们证明，聚合物接枝使蛋白质吸附提高了75%，并增加了细胞在聚合物表面的铺展。由于铺展与细胞黏附和增殖直接相关，我们证明pNaSS接枝促进了PCL的生物相容性。
