Ashkenasy Nurit, Sánchez-Quesada Jorge, Bayley Hagan, Ghadiri M Reza
Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Angew Chem Int Ed Engl. 2005 Feb 18;44(9):1401-4. doi: 10.1002/anie.200462114.
Functional supramolecular chemistry at the single-molecule level. Single strands of DNA can be captured inside α-hemolysin transmembrane pore protein to form single-species α-HL·DNA pseudorotaxanes. This process can be used to identify a single adenine nucleotide at a specific location on a strand of DNA by the characteristic reductions in the α-HL ion conductance. This study suggests that α-HL-mediated single-molecule DNA sequencing might be fundamentally feasible.
单分子水平的功能超分子化学。DNA单链可被捕获在α-溶血素跨膜孔蛋白内,形成单物种α-HL·DNA准轮烷。这一过程可通过α-HL离子电导的特征性降低来识别DNA链上特定位置的单个腺嘌呤核苷酸。该研究表明,α-HL介导的单分子DNA测序从根本上来说可能是可行的。