Allodi S, Cavalcante L A, Hokoç J N, Bernardes R F
Departamento de Neurobiologia, Instituto de Biofísica C. Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
Anat Embryol (Berl). 1992;185(5):489-99. doi: 10.1007/BF00174086.
In this study, we have examined the genesis of neurons of the retinal ganglion cell layer of the opossum Didelphis marsupialis by [3H]-thymidine autoradiography. Our results suggest that most neurons surviving to adulthood are generated in postnatal life from day 1 to day 23. Cells are generated according to a coarse gradient from the retinal geometric center to the periphery. Regional analysis of soma size distributions in different cohorts suggest that this gradient is actually formed by two partially-overlapping, concentric waves of cell proliferation. Most medium and large ganglion cells are formed during the early wave, whereas most displaced amacrine cells and small ganglion cells are formed during the late wave. Our results confirm the appropriateness of the opossum as a model for studies of development of the mammalian visual system.
在本研究中，我们通过[3H] - 胸腺嘧啶核苷放射自显影术研究了负鼠（Didelphis marsupialis）视网膜神经节细胞层神经元的起源。我们的结果表明，大多数存活至成年期的神经元是在出生后第1天到第23天产生的。细胞按照从视网膜几何中心到周边的粗略梯度产生。对不同群体中细胞体大小分布的区域分析表明，这种梯度实际上是由两个部分重叠的同心细胞增殖波形成的。大多数中型和大型神经节细胞在早期波中形成，而大多数移位无长突细胞和小型神经节细胞在晚期波中形成。我们的结果证实了负鼠作为哺乳动物视觉系统发育研究模型的适用性。
