Sengelaub D R, Dolan R P, Finlay B L
J Comp Neurol. 1986 Apr 22;246(4):527-43. doi: 10.1002/cne.902460409.
During the early postnatal period in the hamster, the retinal ganglion cell layer grows, establishes its central connections, and undergoes substantial cell loss. In this study, we describe the development of the retinal ganglion cell layer with particular attention to the creation of local specializations in cell density. Changes in the number and spatial distribution of cells identified by a single 3H thymidine injection were examined through the period of maximal cell loss (postnatal days 4-10) and at adulthood. The cells of the retinal ganglion cell layer are generated from embryonic day 10 to postnatal day 3. Overall, cell number in the ganglion cell layer increases by approximately 108,000 cells (223%) from postnatal day 1 to 5, because of continued migration of cells generated prenatally. Cell number decreases from postnatal day 5 to 10 (25%), coincident with the presence of degenerating cells. Cell type is correlated with day of generation: the largest cells, all having retinal ganglion cell morphology, are generated on embryonic days 10 and 11; intermediate-sized cells predominantly of ganglion cell morphology on embryonic day 12; and smaller cells of displaced amacrine or glial cell morphology thereafter. At adulthood, the hamster retina shows a streaklike elevation of cell density through central retina. However, at the time of maximal cell number (postnatal day 5), cell density is uniform across the retina. During the period of cell degeneration, cells are lost in greater relative numbers from the retinal periphery. This cell loss occurs principally from the first-generated cells (embryonic days 10 and 11), as shown by both changes in the distribution of labeled cells and by the spatial pattern of labeled degenerating cells. From postnatal day 10 to adulthood, relative cell density continues to decline in the periphery of the retina, thus suggesting that differential growth completes the production of the adult cell density distribution.
在仓鼠出生后的早期阶段，视网膜神经节细胞层生长、建立其中心连接，并经历大量细胞丢失。在本研究中，我们描述了视网膜神经节细胞层的发育，特别关注细胞密度局部特化的形成。通过单次注射³H胸腺嘧啶核苷标记细胞，研究了在细胞大量丢失期（出生后第4 - 10天）和成年期细胞数量及空间分布的变化。视网膜神经节细胞层的细胞在胚胎第10天至出生后第3天产生。总体而言，由于产前产生的细胞持续迁移，神经节细胞层的细胞数量从出生后第1天到第5天增加了约108,000个细胞（223%）。细胞数量从出生后第5天到第10天减少（25%），这与退化细胞的出现一致。细胞类型与产生日期相关：最大的细胞，均具有视网膜神经节细胞形态，在胚胎第10天和第11天产生；中等大小的细胞，主要为神经节细胞形态，在胚胎第12天产生；此后是较小的具有移位无长突细胞或神经胶质细胞形态的细胞。成年时，仓鼠视网膜在中央视网膜处呈现出条纹状的细胞密度升高。然而，在细胞数量最多时（出生后第5天），整个视网膜的细胞密度是均匀的。在细胞退化期，视网膜周边相对丢失的细胞数量更多。这种细胞丢失主要发生在最早产生的细胞（胚胎第10天和第11天）中，这通过标记细胞分布的变化以及标记退化细胞的空间模式都得到了证实。从出生后第10天到成年，视网膜周边的相对细胞密度持续下降，这表明差异生长完成了成年细胞密度分布的形成。
