Hishikawa Keiichi, Marumo Takeshi, Miura Shigeki, Nakanishi Asato, Matsuzaki Yumi, Shibata Katsunori, Kohike Hiroko, Komori Takuya, Hayashi Matsuhiko, Nakaki Toshio, Nakauchi Hiromitsu, Okano Hideyuki, Fujita Toshiro
Department of Clinical Renal Regeneration, Graduate School of Medicine, University of Tokyo, Japan.
Biochem Biophys Res Commun. 2005 Mar 4;328(1):288-91. doi: 10.1016/j.bbrc.2004.12.167.
Side population (SP) is reported to be a stem cell-rich population. In the presence of leukemia inhibitory factor (LIF), cultured kidney SP cells differentiated into multi-lineage in collagen gel but not in synthesized polymer that has no cell adhesion factor. In cultured kidney SP cells, gene expression of kidney-specific cadherin 16 was specifically upregulated in collagen gel but not in synthesized polymer. Moreover, decreasing cadherin 16 expression using siRNA abolished LIF-induced multi-lineage differentiation of kidney SP in collagen gel. These results indicated that LIF induced multi-lineage differentiation of adult stem-like cells in kidney via cadherin 16.
据报道，侧群细胞（SP）是富含干细胞的群体。在白血病抑制因子（LIF）存在的情况下，培养的肾SP细胞在胶原凝胶中可分化为多谱系细胞，但在没有细胞粘附因子的合成聚合物中则不能。在培养的肾SP细胞中，肾特异性钙黏蛋白16的基因表达在胶原凝胶中特异性上调，但在合成聚合物中则不然。此外，使用小干扰RNA（siRNA）降低钙黏蛋白16的表达可消除LIF诱导的肾SP细胞在胶原凝胶中的多谱系分化。这些结果表明，LIF通过钙黏蛋白16诱导肾中成年干细胞样细胞的多谱系分化。
