Yamaki Kunihiko, Takiyama Naoaki, Itho Norihiko, Mizuki Nobuhisa, Seiya Maehara, Sinsuke Wakaiki, Hayakawa Kouichi, Kotani Tadao
Department of Ophthalmology, Akita University School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan.
Exp Eye Res. 2005 Feb;80(2):273-80. doi: 10.1016/j.exer.2004.09.010.
We have investigated whether a Vogt-Koyanagi-Harada (VKH)-like disease can be induced in Akita dogs by immunizing them with tyrosinase related protein 1 (TRP1), and compared the alterations induced to those of Akita dogs with a spontaneously occurring disease that resembles human VKH disease. Two Akita dogs were immunized with a peptide mixture of human TRP1. The changes in the eyes were followed by slit-lamp biomicroscopy, ophthalmoscopy, and fluorescein angiography (FA). The eyes, skin, and brains were studied by standard histological methods at about 20 months after the first immunization in one dog (dog 1), and at 3 weeks after the second immunization in the second dog (dog 2). Both dogs developed chorioretinal disease 3-4 weeks after the first immunization. Many inflammatory cells infiltrated into the anterior chamber and anterior vitreous. The fundus showed geographic, multifocal exudative retinal detachments. Multifocal leakages of fluorescein were detected from the choroid. Histologically, exudative retinal detachment was present, and inflammatory cells were seen in the subretinal space in the eyes of dog 2 taken three weeks after the second immunization. The choroid was thickened by the infiltration of inflammatory cells in some lesions. Dalen-Fuchs nodules were seen in the eye of dog 2. Depigmentation, pigment dispersion, and infiltration of many inflammatory cells around hair follicles and vessels were seen in the skin taken three weeks post-immunization. The clinical course and changes in the eyes and skin were very similar to those seen in the Akita dogs with spontaneously occurring VKH disease. We concluded that a VKH-like disease had been induced in these dogs, and this supports the tentative conclusion that the spontaneously occurring chorioretinal disease in Akita dogs is VKH disease.
我们研究了通过用酪氨酸酶相关蛋白1（TRP1）免疫秋田犬，是否能诱导出类似伏格特-小柳-原田（VKH）病的疾病，并将诱导出的改变与患有类似人类VKH病的自发疾病的秋田犬的改变进行比较。两只秋田犬用人类TRP1的肽混合物进行免疫。通过裂隙灯生物显微镜检查、检眼镜检查和荧光素血管造影（FA）来跟踪眼睛的变化。在第一只狗（狗1）首次免疫后约20个月，以及第二只狗（狗2）第二次免疫后3周，通过标准组织学方法对眼睛、皮肤和大脑进行研究。两只狗在首次免疫后3 - 4周都出现了脉络膜视网膜疾病。许多炎性细胞浸润到前房和前部玻璃体。眼底显示地图状、多灶性渗出性视网膜脱离。从脉络膜检测到多灶性荧光素渗漏。组织学上，存在渗出性视网膜脱离，在第二次免疫后3周采集的狗2的眼睛视网膜下间隙可见炎性细胞。在一些病变中，脉络膜因炎性细胞浸润而增厚。在狗2的眼睛中可见达伦-富克斯结节。免疫后3周采集的皮肤可见色素脱失、色素弥散以及毛囊和血管周围许多炎性细胞浸润。眼睛和皮肤的临床病程及变化与患有自发VKH病的秋田犬所见非常相似。我们得出结论，这些狗诱导出了类似VKH病的疾病，这支持了秋田犬自发的脉络膜视网膜疾病是VKH病的初步结论。
