Devreker F, Winston R M, Hardy K
Institute of Obstetrics and Gynaecology, Hospital Erasme, Brussels, Belgium.
Fertil Steril. 1998 Feb;69(2):293-9. doi: 10.1016/s0015-0282(97)00463-9.
To study the effect of glutamine on human preimplantation embryo development in vitro.
A randomized, controlled, prospective study of 138 normally fertilized and nontransferred human embryos. On day 2 after fertilization, the embryos were allocated randomly to glucose-free medium with or without 1 mM of glutamine.
A university hospital-based IVF-ET program.
PATIENT(S): Couples undergoing IVF treatment.
INTERVENTION(S): Embryo culture.
MAIN OUTCOME MEASURE(S): Blastocyst formation, trophectoderm and inner cell mass numbers, and pyruvate uptake and lactate production by individual embryos.
RESULT(S): A higher proportion of embryos reached the morula (89% versus 68%, respectively) and blastocyst (71% versus 54%, respectively) stages when cultured with glutamine compared with embryos cultured without glutamine. Blastocyst cell numbers were not statistically significantly different for embryos grown in the presence or absence of glutamine. Pyruvate uptake and lactate production were higher in the presence of glutamine throughout development; these increases were statistically significant at the blastocyst stage.
CONCLUSION(S): This study demonstrates that supplementation of the culture medium with glutamine is beneficial for human preimplantation embryo development in vitro, increasing the proportion of embryos that develop to the morula and blastocyst stages.
研究谷氨酰胺对人植入前胚胎体外发育的影响。
对138个正常受精且未移植的人胚胎进行随机、对照、前瞻性研究。在受精后第2天，将胚胎随机分配至含或不含1 mM谷氨酰胺的无糖培养基中。
一家大学医院的体外受精 - 胚胎移植项目。
接受体外受精治疗的夫妇。
胚胎培养。
囊胚形成、滋养外胚层和内细胞团数量，以及单个胚胎的丙酮酸摄取和乳酸生成。
与未添加谷氨酰胺培养的胚胎相比，添加谷氨酰胺培养的胚胎有更高比例达到桑葚胚阶段（分别为89%对68%）和囊胚阶段（分别为71%对54%）。在有或无谷氨酰胺环境下生长的胚胎，其囊胚细胞数量在统计学上无显著差异。在整个发育过程中，添加谷氨酰胺时丙酮酸摄取和乳酸生成更高；在囊胚阶段，这些增加具有统计学意义。
本研究表明，在培养基中添加谷氨酰胺有利于人植入前胚胎的体外发育，可增加发育至桑葚胚和囊胚阶段的胚胎比例。
