Pedersen-Bjergaard Stig, Rasmussen Knut Einar
School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Mar 5;817(1):3-12. doi: 10.1016/j.jchromb.2004.08.034.
The demand for automation of liquid-liquid extraction (LLE) in drug analysis combined with the demand for reduced sample preparation time has led to the recent development of liquid-phase microextraction (LPME) based on disposable hollow fibres. In LPME, target drugs are extracted from aqueous biological samples, through a thin layer of organic solvent immobilised within the pores of the wall of a porous hollow fibre, and into an microl volume of acceptor solution inside the lumen of the hollow fibre. After extraction, the acceptor solution is subjected directly to a final analysis either by high performance liquid chromatography (HPLC), capillary electrophoresis (CE), mass spectrometry (MS), or capillary gas chromatography (GC) without any further treatments. Hollow fibre-based LPME may provide high enrichment of drugs and excellent sample clean-up, and probably has a broad application potential within the area of drug analysis. This review focuses on the principle of LPME, and recent applications of three-phase, two-phase, and carrier mediated LPME of drugs from plasma, whole blood, urine, and breast milk.
药物分析中对液液萃取（LLE）自动化的需求，再加上对缩短样品制备时间的需求，促使了基于一次性中空纤维的液相微萃取（LPME）技术的最新发展。在LPME中，目标药物从水性生物样品中通过固定在多孔中空纤维壁孔内的一薄层有机溶剂进行萃取，并进入中空纤维内腔中的微升体积的接受溶液中。萃取后，接受溶液无需任何进一步处理，直接通过高效液相色谱（HPLC）、毛细管电泳（CE）、质谱（MS）或毛细管气相色谱（GC）进行最终分析。基于中空纤维的LPME可以实现药物的高富集和出色的样品净化，并且在药物分析领域可能具有广泛的应用潜力。本综述重点关注LPME的原理，以及三相、两相和载体介导的LPME在从血浆、全血、尿液和母乳中萃取药物方面的最新应用。
