Musteata Mihaela L, Musteata Florin Marcel
Department of Pharmaceutical Sciences, Albany College of Pharmacy and Health Sciences, Albany, NY 12208, USA.
Bioanalysis. 2009 Sep;1(6):1081-102. doi: 10.4155/bio.09.88.
Integration of sampling and sample preparation with various analytical instruments is a highly desirable feature for any analytical method. This is most conveniently achieved by using microextraction techniques or various microdevices. Among these techniques, solid-phase microextraction (SPME) is particularly remarkable due to its simplicity and effectiveness. This review discusses the most recent applications of SPME in bioanalysis, grouped according to the analytical instrument that SPME is coupled to. It is shown that one of the most important aspects of such analytical methods is the ability of SPME to perform direct and selective extraction of analytes from complex biological samples. By far, the most popular method continues to be SPME coupled to GC. Nevertheless, the last 2 years have witnessed significant advances in other areas, such as successful automation of SPME coupled to liquid chromatography and the development of new coatings suitable for direct extraction from biological samples. Furthermore, a few bioanalytical applications based on direct coupling of SPME to MS, ion mobility spectrometry, CE and analytical chemiluminescence have been reported.
将采样和样品前处理与各种分析仪器相结合，是任何分析方法都非常期望具备的特性。通过使用微萃取技术或各种微型装置能最方便地实现这一点。在这些技术中，固相微萃取（SPME）因其简单性和有效性而格外引人注目。本综述讨论了SPME在生物分析中的最新应用，并根据与之联用的分析仪器进行了分类。结果表明，此类分析方法最重要的一个方面是SPME能够直接且选择性地从复杂生物样品中萃取分析物。到目前为止，最常用的方法仍是SPME与气相色谱联用。然而，在过去两年中，其他领域也取得了显著进展，例如SPME与液相色谱联用的成功自动化以及适用于从生物样品中直接萃取的新型涂层的开发。此外，还报道了一些基于SPME与质谱、离子迁移谱、毛细管电泳和分析化学发光直接联用的生物分析应用。
