Zych Krystyna, Perepelov Andrei V, Baranowska Agata, Zabłotni Agnieszka, Knirel Yuriy A, Sidorczyk Zygmunt
Department of General Microbiology, Institute of Microbiology and Immunology, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.
FEMS Immunol Med Microbiol. 2005 Feb 1;43(2):141-8. doi: 10.1016/j.femsim.2004.07.011.
The O-specific polysaccharide of the lipopolysaccharide of Proteus penneri strain 75 consists of tetrasaccharide-ribitol phosphate repeating units and resembles ribitol teichoic acids of Gram-positive bacteria. The following structure of the polysaccharide was elucidated by chemical methods and 1H and 13C NMR spectroscopy: [structure in text] where Rib-ol is ribitol. Serological studies with polyclonal antisera showed that the same structure of the O-polysaccharide occurred in two strains: P. penneri 75 and 128. A similar structure has been established earlier for the O-polysaccharide of P. penneri 103 [Drzewiecka, D., et al., Carbohydr. Res. 337 (2002) 1535-1540]. On the basis of complex serological investigations with use of two polyclonal P. penneri 75 and 103 O-antisera, five strains could be classified into Proteus O73 serogroup: P. penneri 48, 75, 90, 103 and 128, two of which (P. penneri 75 and 128) should be subdivided into subgroup 73a, 73b and three others (P. penneri 48, 90 and 103) into subgroup 73a, 73c. Epitopes responsible for the cross-reactivity of P. penneri O73 strains and a related strain of P. mirabilis O20 were tentatively defined.
彭氏变形杆菌75株脂多糖的O-特异性多糖由四糖-核糖醇磷酸重复单元组成，类似于革兰氏阳性菌的核糖醇磷壁酸。通过化学方法以及1H和13C核磁共振光谱确定了该多糖的以下结构：[文中结构]，其中Rib-ol为核糖醇。用多克隆抗血清进行的血清学研究表明，O-多糖的相同结构存在于两株菌中：彭氏变形杆菌75和128。此前已确定彭氏变形杆菌103株的O-多糖具有类似结构[德泽维茨卡，D.等人，《碳水化合物研究》337 (2002) 1535 - 1540]。基于使用两株彭氏变形杆菌75和103的O-多克隆抗血清进行的复杂血清学研究，五株菌可被归类为变形杆菌O73血清群：彭氏变形杆菌48、75、90、103和128，其中两株（彭氏变形杆菌75和128）应细分为73a亚组、73b亚组，另外三株（彭氏变形杆菌48、90和103）细分为73a亚组、73c亚组。初步确定了负责彭氏变形杆菌O73菌株与奇异变形杆菌O20相关菌株交叉反应性的表位。
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