Zych Krystyna, Siwińska Małgorzata, Sidorczyk Zygmunt
Department of General Microbiology, Institute of Microbiology and Immunology, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.
Arch Immunol Ther Exp (Warsz). 2005 Nov-Dec;53(6):540-5.
Gram-negative bacteria of genus Proteus are common human intestinal and urinary tract pathogens. In the genus Proteus there are four clinically important named species: P. mirabilis, P. vulgaris, P. penneri, and P. hauseri, and three unnamed Proteus genomospecies: 4, 5, and 6. The clinical significance of P. penneri, described in 1982 as a new species, is poorly documented. The aim of this work is serological characterization and classification of a ceftriaxone-susceptible P. penneri S29 strain isolated from a 34-year-old patient with postneurosurgical meningitis. In this characterization we will also include a ceftriaxonresistant strain, P. penneri R15, isolated from the same patient after 12 days' treatment with ceftriaxon and other antibiotics.
MATERIAL/METHODS: Rabbit polyclonal O-antisera were obtained against these two strains and purified and lipopolysaccharides (LPS) were extracted from the bacterial mass of the P. penneri S29 and R15 strains. In the serological investigations the following tests were used: enzyme immunosorbent assay (EIA), passive immunohemolysis (PIH), inhibition of these tests, absorption of rabbit O-antisera with the respective LPS, and repeated PIH, SDS/PAGE, and Western blot techniques.
The serological studies of the LPS extracted from both P. penneri strains showed the identity of both preparations of O-polysaccharides from LPS. In P. penneri S29 O-antiserum, four different types of antibodies were described and characterized.
Both investigated P. penneri S29 and R15 strains were classified to the Proteus O31ab serogroup.
变形杆菌属革兰氏阴性菌是常见的人类肠道和泌尿道病原体。变形杆菌属中有四种具有临床重要意义的命名菌种：奇异变形杆菌、普通变形杆菌、彭氏变形杆菌和豪氏变形杆菌，以及三种未命名的变形杆菌基因组种：基因组种4、5和6。1982年被描述为新物种的彭氏变形杆菌的临床意义记录较少。这项工作的目的是对从一名34岁神经外科术后脑膜炎患者分离出的对头孢曲松敏感的彭氏变形杆菌S29菌株进行血清学特征分析和分类。在该特征分析中，我们还将纳入一株头孢曲松耐药菌株，即彭氏变形杆菌R15，它是在患者接受头孢曲松和其他抗生素治疗12天后从同一患者分离得到的。
材料/方法：制备针对这两种菌株的兔多克隆O抗血清，并从彭氏变形杆菌S29和R15菌株的菌团中提取和纯化脂多糖（LPS）。在血清学研究中使用了以下检测方法：酶免疫吸附测定（EIA）、被动免疫溶血（PIH）、这些检测的抑制、用相应的LPS吸收兔O抗血清、重复PIH、SDS/PAGE和蛋白质印迹技术。
对从两种彭氏变形杆菌菌株中提取的LPS进行的血清学研究表明，两种LPS的O多糖制剂具有一致性。在彭氏变形杆菌S29 O抗血清中，描述并鉴定了四种不同类型的抗体。
所研究的彭氏变形杆菌S29和R15菌株均被归类为变形杆菌O31ab血清群。
