匈牙利患与未患卡波西肉瘤的HIV阳性患者中人类疱疹病毒8型感染的患病率

Prevalence of human herpesvirus-8 infection in HIV-positive patients with and without Kaposi's sarcoma in Hungary.

作者信息

Szalai Erika, Gerlei Zsuzsanna, Szlávik János, Szládek Györgyi, Patel Ravi, Hunyadi János, Gergely Lajos, Juhász Attila

机构信息

Department of Microbiology, St. László Hospital, Gyáli u. 5-7, 1097 Budapest, Hungary.

出版信息

FEMS Immunol Med Microbiol. 2005 Feb 1;43(2):265-8. doi: 10.1016/j.femsim.2004.08.012.

DOI:10.1016/j.femsim.2004.08.012

PMID:15681157

Abstract

Human herpesvirus-8 (HHV-8) infection of 130 Hungarian HIV-positive individuals with or without Kaposi's sarcoma was investigated from 158 serum and 122 peripheral blood samples using anti-latency-associated nuclear antigen (LANA) indirect immunofluorescence assay (IFA), recombinant orf65 and orfK8.1 antigen enzyme-linked immunosorbent assays (ELISAs), Western blot assays and orf26 specific nested polymerase chain reaction (PCR). The overall prevalence of HHV-8 infection was found to be 31.5% (41/130) among the Hungarian HIV-positive patients. This seroprevalence rate is 7-11-fold higher than that of healthy HIV-negative blood donors in Hungary. The highest prevalence of HHV-8 infection (36.1%, 35/97) was observed in homo- or bisexual patients. Similar to the serologic results, HHV-8 DNA was not always detectable in all serial samples previously shown to be positive for HHV-8 DNA.

摘要

利用抗潜伏相关核抗原（LANA）间接免疫荧光测定法（IFA）、重组orf65和orfK8.1抗原酶联免疫吸附测定法（ELISA）、蛋白质印迹测定法以及orf26特异性巢式聚合酶链反应（PCR），对130名患有或未患有卡波西肉瘤的匈牙利HIV阳性个体的158份血清样本和122份外周血样本进行了人类疱疹病毒8型（HHV-8）感染情况的调查。结果发现，在匈牙利HIV阳性患者中，HHV-8感染的总体患病率为31.5%（41/130）。这一血清阳性率比匈牙利健康的HIV阴性献血者高出7至11倍。在同性恋或双性恋患者中观察到HHV-8感染的患病率最高（36.1%，35/97）。与血清学结果相似，在先前显示HHV-8 DNA呈阳性的所有系列样本中，并非总能检测到HHV-8 DNA。