Orthopedic Department, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, People's Republic of China.
J Biomed Mater Res B Appl Biomater. 2011 May;97(2):224-34. doi: 10.1002/jbm.b.31804. Epub 2011 Mar 9.
Silicon-incorporated TiO(2) coating (Si-TiO(2) ) was prepared on titanium (Ti) by micro-arc oxidation (MAO) technique in the Ca, P, Si-containing electrolyte. The surface topography, phase, and element composition of the coatings were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy-dispersive X-ray spectrometry (EDS), respectively. Osteoblast-like MC3T3-E1 cells were cultured on the surface of the coatings to evaluate their adhesion behavior. The obtained results showed that Si element was successfully incorporated into the porous TiO(2) coating, which did not alter apparently the surface topography and phase composition of the coating. The adhesion of the MC3T3-E1 cells on Si-incorporated TiO(2) coating was significantly enhanced compared with the Si-free TiO(2) coating and pure Ti plates. In addition, the enhanced cell adhesion may at least partly be mediated by integrin β1-FAK signal transduction pathway. The present work suggests that the Si-TiO(2) coating is worth further consideration for orthopedic implant applications.
硅掺杂二氧化钛（Si-TiO2）涂层通过微弧氧化（MAO）技术在含有钙、磷、硅的电解液中在钛（Ti）上制备。使用扫描电子显微镜（SEM）、X 射线衍射（XRD）和能谱仪（EDS）分别对涂层的表面形貌、相和元素组成进行了表征。将成骨样 MC3T3-E1 细胞培养在涂层表面，以评估其黏附行为。结果表明，硅元素成功地掺入了多孔 TiO2 涂层中，这并没有明显改变涂层的表面形貌和相组成。与无硅 TiO2 涂层和纯钛板相比，MC3T3-E1 细胞在掺硅 TiO2 涂层上的黏附明显增强。此外，增强的细胞黏附至少部分可能是通过整合素 β1-FAK 信号转导途径介导的。本工作表明，硅掺杂二氧化钛涂层在骨科植入物应用中值得进一步考虑。
