Cheng Sen-Sung, Lin Huang-Yuan, Chang Shang-Tzen
School of Forestry and Resource Conservation, National Taiwan University, Taipei 106, Taiwan.
J Agric Food Chem. 2005 Feb 9;53(3):614-9. doi: 10.1021/jf0484529.
In this study antifungal activities of essential oils from different tissues of Japanese cedar (Cryptomeria japonica D. Don) against four wood decay fungi and six tree pathogenic fungi were investigated. In addition, the yields of essential oils obtained by water distillation were compared and their constituents determined by GC-MS analyses. The yield of essential oils from four tissues of Japanese cedar is in the decreasing order of leaf (27.38 mL/kg) > bark (6.31 mL/kg) > heartwood (3.80 mL/kg) > sapwood (1.27 mL/kg). Results obtained from the antifungal tests demonstrate that the essential oil of Japanese cedar heartwood used against Laetiporus sulphureus and Trametes versicolor and sapwood essential oil used against L. sulphureus had strong antifungal activities at 500 mug/mL, with IC(50) values of 39, 91, and 94 microg/mL, respectively. Besides, the essential oils of Japanese cedar heartwood used against Rhizoctonia solani, Collectotrichum gloeosporioides, Fusarium solani, and Ganoderma australe had strong antifungal activities at 500 microg/mL, with IC(50) values of 65, 80, 80, and 110 microg/mL, respectively. GC-MS analyses showed that the sesquiterpene hydrocarbon compounds dominate in the essential oil from Japanese cedar heartwood, amounting to a total percentage of 82.56%, with the major compounds of delta-cadinene (18.60%), isoledene (12.41%), and gamma-muurolene (11.82%). It is proposed that the excellent antifungal activities of Japanese cedar heartwood essential oils might correlate with the presence of these compounds.
本研究调查了日本柳杉(Cryptomeria japonica D. Don)不同组织的精油对四种木材腐朽真菌和六种树木致病真菌的抗真菌活性。此外,还比较了水蒸馏法获得的精油产量,并通过气相色谱 - 质谱联用(GC - MS)分析确定了其成分。日本柳杉四种组织的精油产量由高到低依次为:叶(27.38 mL/kg)>树皮(6.31 mL/kg)>心材(3.80 mL/kg)>边材(1.27 mL/kg)。抗真菌试验结果表明,日本柳杉心材精油对硫黄多孔菌(Laetiporus sulphureus)和云芝(Trametes versicolor)以及边材精油对硫黄多孔菌在500 μg/mL时具有较强的抗真菌活性,IC(50)值分别为39、91和94 μg/mL。此外,日本柳杉心材精油对立枯丝核菌(Rhizoctonia solani)、炭疽菌(Collectotrichum gloeosporioides)、茄腐镰刀菌(Fusarium solani)和南方灵芝(Ganoderma australe)在500 μg/mL时也具有较强的抗真菌活性,IC(50)值分别为65、80、80和110 μg/mL。GC - MS分析表明,日本柳杉心材精油中倍半萜烃类化合物占主导,总含量为82.56%,主要化合物为δ-杜松烯(18.60%)、异丁子香烯(12.41%)和γ-依兰油烯(11.82%)。据推测,日本柳杉心材精油优异的抗真菌活性可能与这些化合物的存在有关。