Chang Hui-Ting, Cheng Ying-Hung, Wu Chi-Lin, Chang Shang-Tzen, Chang Tun-Tschu, Su Yu-Chang
School of Forestry and Resource Conservation, National Taiwan University, Taipei 106, Taiwan.
Bioresour Technol. 2008 Sep;99(14):6266-70. doi: 10.1016/j.biortech.2007.12.005. Epub 2008 Feb 21.
Resistance to conventional fungicides causes the poor disease control of agriculture. Natural products from plants have great potential as novel fungicide sources for controlling pathogenic fungi. In this study antipathogenic activity of the leaf essential oil and its constituents from Calocedrus macrolepis var. formosana Florin were evaluated in vitro against six plant pathogenic fungi. Chemical analysis of leaf oil by GC/MS allowed identification of alpha-pinene (44.2%), limonene (21.6%), beta-myrcene (8.9%), beta-caryophyllene (8.2%), caryophyllene oxide (2.4%), alpha-cadinol (1.6%), beta-pinene (1.2%), and T-muurolol (1.1%) as main components. Sesquiterpenoid components of the oil were more effective than monoterpenoid components of the oil. In particular, T-muurolol and alpha-cadinol strongly inhibited the growth of Rhizoctonia solani and Fusarium oxysporum, with the IC(50) values < 50 microg ml(-1). These compounds also efficiently inhibited the mycelial growths of Colletotrichum gloeosporioides, P. funerea, Ganoderma australe and F. solani. These results showed that T-muurolol and alpha-cadinol possess antifungal activities against a broad spectrum of tested plant pathogenic fungi and could be used as potential antifungal agents for the control of fungal diseases in plants.
对传统杀菌剂产生抗性导致农业病害防治效果不佳。植物天然产物作为控制致病真菌的新型杀菌剂来源具有巨大潜力。在本研究中，对台湾油杉叶精油及其成分对六种植物致病真菌的体外抗病原活性进行了评估。通过气相色谱/质谱联用仪对叶油进行化学分析，鉴定出主要成分包括α-蒎烯（44.2%）、柠檬烯（21.6%）、β-月桂烯（8.9%）、β-石竹烯（8.2%）、石竹烯氧化物（2.4%）、α-杜松醇（1.6%）、β-蒎烯（1.2%）和T-穆罗醇（1.1%）。该油的倍半萜类成分比单萜类成分更有效。特别是，T-穆罗醇和α-杜松醇强烈抑制茄丝核菌和尖孢镰刀菌的生长，IC(50)值<50微克/毫升(-1)。这些化合物还能有效抑制炭疽菌、灰葡萄孢菌、南方灵芝和茄腐镰刀菌的菌丝生长。这些结果表明，T-穆罗醇和α-杜松醇对多种测试植物致病真菌具有抗真菌活性，可作为控制植物真菌病害的潜在抗真菌剂。
