[丁香假单胞菌番茄致病变种及基因组种4其他菌株O-多糖的结构多样性与血清学分类]

[Structural diversity of O-polysaccharides and serological classification of Pseudomonas syringae pv. garcae and other strains of genomospecies 4].

作者信息

Ovod V V, Zdorovenko E L, Shashkov A S, Kocharova N A, Knirel' Iu A

出版信息

Mikrobiologiia. 2004 Nov-Dec;73(6):777-89.

Abstract

Novel O-serotypes were revealed among Pseudomonas syringae pv. garcae strains by using a set of mouse monoclonal antibodies specific to the lipopolysaccharide O-polysaccharide. Structural studies showed that the O-polysaccharide of P. syringae pv. garcae NCPPB 2708 is a hitherto unknown linear L-rhamnan lacking strict regularity and having two oligosaccharide repeating units I and II, which differ in the position of substitution in one of the rhamnose residues and have the following structures: I: --> 3)-alpha-L-Rha-(1 --> 2)-alpha-L-Rha-(1 --> 2)-alpha-L-Rha-(1 --> 3)-alpha-L-Rha-(1 -->; II: --> 3)-alpha-L-Rha-(1 --> 3)-alpha-L-Rha-(1 --> 2)-alpha-L-Rha-(1 --> 3)-alpha-L-Rha-(1 -->. The branched polysaccharides of P. syringae pv. garcae ICMP 8047 and NCPPB 588 have the same L-rhamnan backbone with repeating units I and II and a lateral chain of (alpha1 --> 4)- or (alpha1 --> 3)-linked residues of 3-acetamido-3,6-dideoxy-D-galactose (D-Fuc3NAc). Several monoclonal antibody epitopes associated with the L-rhamnan backbone or the lateral alpha-D-Fuc3NAc residues were characterized.

摘要

利用一组对脂多糖O-多糖具有特异性的小鼠单克隆抗体，在丁香假单胞菌番茄致病变种菌株中发现了新的O-血清型。结构研究表明，丁香假单胞菌番茄致病变种NCPPB 2708的O-多糖是一种迄今未知的线性L-鼠李聚糖，缺乏严格的规律性，有两个寡糖重复单元I和II，它们在一个鼠李糖残基的取代位置上有所不同，结构如下：I：→3)-α-L-鼠李糖-(1→2)-α-L-鼠李糖-(1→2)-α-L-鼠李糖-(1→3)-α-L-鼠李糖-(1→；II：→3)-α-L-鼠李糖-(1→3)-α-L-鼠李糖-(1→2)-α-L-鼠李糖-(1→3)-α-L-鼠李糖-(1→。丁香假单胞菌番茄致病变种ICMP 8047和NCPPB 588的支链多糖具有相同的带有重复单元I和II的L-鼠李糖主链以及由(α1→4)-或(α1→3)-连接的3-乙酰氨基-3,6-二脱氧-D-半乳糖（D-Fuc3NAc）残基组成的侧链。对与L-鼠李糖主链或侧链α-D-Fuc3NAc残基相关的几个单克隆抗体表位进行了表征。