Zdorovenko E L, Zatonsky G V, Zdorovenko G M, Pasichnyk L A, Shashkov A S, Knirel Y A
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospeckt 47, 119991 Moscow, Russia.
Carbohydr Res. 2001 Dec 7;336(4):329-36. doi: 10.1016/s0008-6215(01)00268-3.
Studies by sugar and methylation analyses, Smith degradation, and 1H and 13C NMR spectroscopy revealed a structural heterogeneity in the O-polysaccharides of Pseudomonas syringae pvs. coronafaciens IMV 9030 and atrofaciens IMV 8281 owing to the presence of different types of repeating units. In strain IMV 9030, the major repeating units are a linear alpha-L-rhamnose trisaccharide and a tetrasaccharide (A, n=0 or 1). A minor repeating unit is a branched pentasaccharide with an alpha-L-rhamnose main chain and a lateral 3-acetamido-3,6-dideoxy-D-galactose (D-Fuc3NAc) residue (B, X=2, n=1). In strain IMV 8281, all repeating units are branched and differ in size and position of substitution of one of the alpha-L-rhamnose residues (tetrasaccharide, B, X=3, n=0; pentasaccharides, B, X=2 or 3, n=1). [structure--see text] Reinvestigation of the structure of the branched O-polysaccharide of P. syringae pv. tomato IPGR 140 showed that, together with the major tetrasaccharide repeating unit (B, X=3, n=0) [Knirel, Y. A., et al. Carbohydr. Res. 1993, 243, 199-204], it has a minor pentasaccharide repeating unit (B, X=3, n=1).
通过糖和甲基化分析、史密斯降解以及1H和13C核磁共振光谱研究表明，丁香假单胞菌丁香致病变种IMV 9030和萎蔫致病变种IMV 8281的O-多糖存在结构异质性，这是由于存在不同类型的重复单元。在菌株IMV 9030中，主要的重复单元是线性α-L-鼠李糖三糖和四糖（A，n = 0或1）。一个次要的重复单元是具有α-L-鼠李糖主链和侧链3-乙酰氨基-3,6-二脱氧-D-半乳糖（D-Fuc3NAc）残基的分支五糖（B，X = 2，n = 1）。在菌株IMV 8281中，所有重复单元都是分支的，并且在其中一个α-L-鼠李糖残基的取代大小和位置上有所不同（四糖，B，X = 3，n = 0；五糖，B，X = 2或3，n = 1）。[结构 - 见正文]对丁香假单胞菌番茄致病变种IPGR 140的分支O-多糖结构的重新研究表明，除了主要的四糖重复单元（B，X = 3，n = 0）[Knirel，Y. A.等人，Carbohydr. Res. 1993，243，199 - 204]之外，它还有一个次要的五糖重复单元（B，X = 3，n = 1）。
