Ovod V V, Zdorovenko E L, Shashkov A S, Kocharova N A, Knirel Y A
Institute of Medical Technology, University of Tampere, Finland.
Eur J Biochem. 2000 Apr;267(8):2372-9. doi: 10.1046/j.1432-1327.2000.01250.x.
The O polysaccharide (OPS) moiety of the lipopolysaccharide (LPS) of a phytopathogenic bacterium Pseudomonas syringae pv. ribicola NCPPB 1010 was studied by sugar and methylation analyses, Smith degradation, and 1H- and 13C-NMR spectroscopy, including 2D COSY, TOCSY, NOESY and H-detected 1H,13C HMQC experiments. The OPS structure was elucidated, and shown to be composed of branched pentasaccharide repeating units (O repeats) of two types, major (1) and minor (2), differing in the position of substitution of one of the rhamnose residues. Both O repeats form structurally homogeneous blocks within the same polysaccharide molecule. Although P. syringae pv. ribicola NCPPB 1010 demonstrates genetic relatedness and similarity in the OPS chemical structure to some other P. syringae pathovars, it did not cross-react with any OPS-specific mAbs produced against heterologous P. syringae strains. Therefore, we propose to classify P. syringae pv. ribicola NCPPB 1010 in a new serogroup, O8.
利用糖分析、甲基化分析、史密斯降解以及一维和二维核磁共振波谱（包括二维化学位移相关谱、全相关谱、核欧沃豪斯效应谱和氢检测的异核多量子相干实验），对植物致病细菌丁香假单胞菌丁香致病变种（Pseudomonas syringae pv.）ribicola NCPPB 1010脂多糖（LPS）的O多糖（OPS）部分进行了研究。阐明了OPS结构，结果表明其由两种类型的分支五糖重复单元（O重复单元）组成，即主要的（1）和次要的（2），二者鼠李糖残基之一的取代位置不同。两种O重复单元在同一多糖分子内形成结构均一的区域。虽然丁香假单胞菌丁香致病变种ribicola NCPPB 1010在OPS化学结构上与其他一些丁香假单胞菌致病型具有遗传相关性和相似性，但它与针对异源丁香假单胞菌菌株产生的任何OPS特异性单克隆抗体均无交叉反应。因此，我们建议将丁香假单胞菌丁香致病变种ribicola NCPPB 1010归为一个新的血清群，即O8。
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