丁香假单胞菌丁香致病变种NCPPB 1879(T)的O-多糖结构，其具有3-乙酰氨基-3,6-二脱氧-D-半乳糖残基侧链。

Structure of the O-polysaccharide of Pseudomonas syringae pv. delphinii NCPPB 1879(T) having side chains of 3-acetamido-3,6-dideoxy-D-galactose residues.

作者信息

Zdorovenko E L, Zatonsky G V, Kocharova N A, Shashkov A S, Knirel Y A, Ovod V V

机构信息

Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 119991, Russia.

出版信息

Biochemistry (Mosc). 2002 May;67(5):558-65. doi: 10.1023/a:1015550312615.

DOI:10.1023/a:1015550312615

PMID:12059776

Abstract

The O-polysaccharide (OPS) was obtained from the lipopolysaccharide of Pseudomonas syringae pv. delphinii NCPPB 1879(T) and studied by sugar and methylation analyses, Smith degradation, and (1)H- and (13)C-NMR spectroscopy. The OPS was found to contain residues of L-rhamnose (L-Rha) and 3-acetamido-3,6-dideoxy-D-galactose (D-Fuc3NAc), and the following structure of the major (n = 2) and minor (n = 3) heptasaccharide repeating units of the OPS was established: [carbohydrate structure: see text]. The OPS is distinguished by the presence of oligosaccharide side chains consisting of three D-Fuc3NAc residues that are connected to each other by the (alpha 1-->2)-linkage. The OPS is characterized by a structural heterogeneity due to a different position of substitution of one of the four L-rhamnose residues in the main chain of the repeating unit as well as to the presence of oligosaccharide units with an incomplete side chain.

摘要

O-多糖（OPS）是从丁香假单胞菌丁香致病变种NCPPB 1879(T)的脂多糖中获得的，并通过糖分析、甲基化分析、史密斯降解以及¹H-和¹³C-核磁共振光谱进行了研究。发现该OPS含有L-鼠李糖（L-Rha）和3-乙酰氨基-3,6-二脱氧-D-半乳糖（D-Fuc3NAc）残基，并确定了OPS主要（n = 2）和次要（n = 3）七糖重复单元的以下结构：[碳水化合物结构：见正文]。该OPS的特点是存在由三个通过（α1→2）-连接相互连接的D-Fuc3NAc残基组成的寡糖侧链。由于重复单元主链中四个L-鼠李糖残基之一的取代位置不同以及存在侧链不完全的寡糖单元，该OPS具有结构异质性。