Koch W H, Cebula T A, Foster P L, Eisenstadt E
Molecular Biology Branch, Food and Drug Administration, Washington, D.C. 20204.
J Bacteriol. 1992 May;174(9):2809-15. doi: 10.1128/jb.174.9.2809-2815.1992.
We investigated the role of the umuDC and samAB operons in the UV mutability of Salmonella typhimurium. umuDC is located on the chromosome, whereas samAB resides on the virulence plasmid pSLT. Using allele replacement and plasmid curing techniques, we found that UV mutability was eliminated when any of three different umuDC alleles (umuD1, umuC1, or umuD1 umuC1) were on the chromosome even when samAB was present. We conclude that samAB normally does not complement umuDC function in S. typhimurium.
我们研究了umuDC和samAB操纵子在鼠伤寒沙门氏菌紫外线诱变中的作用。umuDC位于染色体上,而samAB位于毒力质粒pSLT上。使用等位基因替换和质粒消除技术,我们发现当三种不同的umuDC等位基因(umuD1、umuC1或umuD1 umuC1)中的任何一个位于染色体上时,即使存在samAB,紫外线诱变也会消除。我们得出结论,在鼠伤寒沙门氏菌中,samAB通常不能补充umuDC的功能。