supX基因在鼠伤寒沙门氏菌紫外线诱导诱变中的作用。
Role of the supX gene in ultraviolet light-induced mutagenesis in Salmonella typhimurium.
作者信息
Overbye K M, Margolin P
出版信息
J Bacteriol. 1981 Apr;146(1):170-8. doi: 10.1128/jb.146.1.170-178.1981.
Abstract
Salmonella typhimurium strains with supX mutations are more sensitive than wild type to killing by ultraviolet (UV) irradiation. Studies with strains bearing the leuD21 mutation revealed that inactivation of the supX locus by a nonsense mutation or a deletion results in a complete lack of ability to produce induced Leu+ reversion mutations after UV irradiation. Suppression of the nonsense supX mutation or the presence of an Escherichia coli K-12 F'-borne supX+ allele restored the capacity for induced reversions and increased cell survival after UV irradiation. Introduction of plasmid pKM101 into supX mutant strains also restored their capacity for UV mutagenesis as well as increased survival. The possible nature of the supX gene product and mechanisms by which it may affect expression of the inducible SOS error-prone repair system are considered.
摘要
带有supX突变的鼠伤寒沙门氏菌菌株比野生型对紫外线（UV）照射杀伤更敏感。对带有leuD21突变的菌株的研究表明，无义突变或缺失使supX基因座失活会导致在紫外线照射后完全丧失产生诱导型Leu +回复突变的能力。无义supX突变的抑制或大肠杆菌K - 12 F'携带的supX +等位基因的存在恢复了诱导回复的能力，并增加了紫外线照射后的细胞存活率。将质粒pKM101导入supX突变菌株也恢复了它们的紫外线诱变能力以及提高了存活率。文中考虑了supX基因产物的可能性质及其可能影响诱导型SOS易错修复系统表达的机制。
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本文引用的文献
1
ANALYSIS OF UNLINKED SUPPRESSORS OF AN O degrees MUTATION IN SALMONELLA.鼠伤寒沙门氏菌O度突变非连锁抑制子的分析
Proc Natl Acad Sci U S A. 1963 Jul;50(1):140-8. doi: 10.1073/pnas.50.1.140.
2
Genetic fine structure of the leucine operon in Salmonella.沙门氏菌中亮氨酸操纵子的遗传精细结构
Genetics. 1963 Mar;48(3):441-57. doi: 10.1093/genetics/48.3.441.
3
[A simple apparatus for determination of the ultraviolet ray output by germicidal lamps].[一种用于测定杀菌灯紫外线输出量的简易装置]
Ann Inst Pasteur (Paris). 1953 Aug;85(2):175-84.
4
Studies of mutability in nutritionally deficient strains of Escherichia coli. I. Genetic analysis of five auxotrophic strains.大肠杆菌营养缺陷型菌株的突变性研究。I. 五个营养缺陷型菌株的遗传分析。
J Bacteriol. 1953 Jan;65(1):27-36. doi: 10.1128/jb.65.1.27-36.1953.
5
Locations of the opp and supX genes of Salmonella typhimurium and Escherichia coli.鼠伤寒沙门氏菌和大肠杆菌opp及supX基因的位置。
J Bacteriol. 1980 Aug;143(2):747-52. doi: 10.1128/jb.143.2.747-752.1980.
6
Effect of ssbA1 and lexC113 mutations on lambda prophage induction, bacteriophage growth, and cell survival.ssbA1和lexC113突变对λ原噬菌体诱导、噬菌体生长及细胞存活的影响。
J Bacteriol. 1980 Aug;143(2):887-96. doi: 10.1128/jb.143.2.887-896.1980.
7
Expression of the leucine operon.亮氨酸操纵子的表达。
J Bacteriol. 1966 Apr;91(4):1570-6. doi: 10.1128/jb.91.4.1570-1576.1966.
8
Genetic analysis of pyrimidine mutants of Salmonella typhimurium.鼠伤寒沙门氏菌嘧啶突变体的遗传分析。
Genetics. 1965 Sep;52(3):643-51. doi: 10.1093/genetics/52.3.643.
9
Interaction between DNA and an Escherichia coli protein omega.DNA与大肠杆菌蛋白ω之间的相互作用。
J Mol Biol. 1971 Feb 14;55(3):523-33. doi: 10.1016/0022-2836(71)90334-2.
10
Operator constitutive mutations in the leucine operon of Salmonella typhimurium.鼠伤寒沙门氏菌亮氨酸操纵子中的操纵基因组成型突变。
Genetics. 1969 Apr;61(4):777-87. doi: 10.1093/genetics/61.4.777.
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