Dowden S B, Strike P
Mol Gen Genet. 1982;186(1):140-4. doi: 10.1007/BF00422926.
Recombinant plasmids which render their host less mutable and more sensitive to some DNA-damaging agents have been isolated from the N-group plasmid R46. These plasmids have been physically mapped and found to originate from the region of R46 that has been deleted in pKM101. This deleted region is well removed from the muc region of R46 and pKM101 which is responsible for the mutator effects of these plasmids. The effect of these anti-mutagenic plasmids on the ability of pKM101 to complement umuC mutations has been examined, and they have been found to inhibit the complementation of such strains. We propose that these plasmids may code for a negative control function acting on the muc gene.
已从N组质粒R46中分离出一些重组质粒，这些重组质粒可使宿主的变异性降低，并对某些DNA损伤剂更敏感。这些质粒已进行物理图谱分析，发现它们起源于R46中在pKM101中已缺失的区域。该缺失区域与R46和pKM101的muc区域相距甚远，而muc区域是这些质粒产生诱变效应的原因。已检测了这些抗诱变质粒对pKM101互补umuC突变能力的影响，发现它们可抑制此类菌株的互补作用。我们提出，这些质粒可能编码一种作用于muc基因的负调控功能。
