顺乌头酸酶将代谢调节与线粒体DNA维持联系起来。

Aconitase couples metabolic regulation to mitochondrial DNA maintenance.

作者信息

Chen Xin Jie, Wang Xiaowen, Kaufman Brett A, Butow Ronald A

机构信息

Department of Molecular Biology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390-9148, USA.

出版信息

Science. 2005 Feb 4;307(5710):714-7. doi: 10.1126/science.1106391.

DOI:10.1126/science.1106391

PMID:15692048

Abstract

Mitochondrial DNA (mtDNA) is essential for cells to maintain respiratory competency and is inherited as a protein-DNA complex called the nucleoid. We have identified 22 mtDNA-associated proteins in yeast, among which is mitochondrial aconitase (Aco1p). We show that this Krebs-cycle enzyme is essential for mtDNA maintenance independent of its catalytic activity. Regulation of ACO1 expression by the HAP and retrograde metabolic signaling pathways directly affects mtDNA maintenance. When constitutively expressed, Aco1p can replace the mtDNA packaging function of the high-mobility-group protein Abf2p. Thus, Aco1p may integrate metabolic signals and mtDNA maintenance.

摘要

线粒体DNA（mtDNA）对于细胞维持呼吸功能至关重要，并且作为一种称为类核的蛋白质 - DNA复合物进行遗传。我们在酵母中鉴定出了22种与mtDNA相关的蛋白质，其中包括线粒体乌头酸酶（Aco1p）。我们发现这种三羧酸循环酶对于mtDNA的维持至关重要，且与其催化活性无关。HAP和逆行代谢信号通路对ACO1表达的调节直接影响mtDNA的维持。当组成型表达时，Aco1p可以替代高迁移率族蛋白Abf2p的mtDNA包装功能。因此，Aco1p可能整合代谢信号和mtDNA的维持。