酵母顺乌头酸酶结合并为线粒体DNA提供代谢偶联保护。
Yeast aconitase binds and provides metabolically coupled protection to mitochondrial DNA.
作者信息
Chen Xin Jie, Wang Xiaowen, Butow Ronald A
机构信息
Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA.
出版信息
Proc Natl Acad Sci U S A. 2007 Aug 21;104(34):13738-43. doi: 10.1073/pnas.0703078104. Epub 2007 Aug 14.
Abstract
Aconitase (Aco1p) is a multifunctional protein: It is an enzyme of the tricarboxylic acid cycle. In animal cells, Aco1p also is a cytosolic protein binding to mRNAs to regulate iron metabolism. In yeast, Aco1p was identified as a component of mtDNA nucleoids. Here we show that yeast Aco1p protects mtDNA from excessive accumulation of point mutations and ssDNA breaks and suppresses reductive recombination of mtDNA. Aconitase binds to both ds- and ssDNA, with a preference for GC-containing sequences. Therefore, mitochondria are opportunistic organelles that seize proteins, such as metabolic enzymes, for construction of the nucleoid, an mtDNA maintenance/segregation apparatus.
摘要
顺乌头酸酶（Aco1p）是一种多功能蛋白质：它是三羧酸循环中的一种酶。在动物细胞中，Aco1p也是一种与mRNA结合以调节铁代谢的胞质蛋白。在酵母中，Aco1p被鉴定为线粒体DNA类核的一个组成部分。在这里我们表明，酵母Aco1p可保护线粒体DNA免受点突变和单链DNA断裂的过度积累，并抑制线粒体DNA的还原重组。顺乌头酸酶可与双链和单链DNA结合，更倾向于含GC的序列。因此，线粒体是机会主义细胞器，会利用诸如代谢酶等蛋白质来构建类核，即一种线粒体DNA维持/分离装置。
相似文献
1
Yeast aconitase binds and provides metabolically coupled protection to mitochondrial DNA.
Proc Natl Acad Sci U S A. 2007 Aug 21;104(34):13738-43. doi: 10.1073/pnas.0703078104. Epub 2007 Aug 14.
2
Aconitase couples metabolic regulation to mitochondrial DNA maintenance.
Science. 2005 Feb 4;307(5710):714-7. doi: 10.1126/science.1106391.
3
Mitochondrial DNA, aconitase 'wraps' it up.
Trends Biochem Sci. 2005 Jun;30(6):294-6. doi: 10.1016/j.tibs.2005.04.007.
4
Irc3 is a mitochondrial DNA branch migration enzyme.
Sci Rep. 2016 May 19;6:26414. doi: 10.1038/srep26414.
5
Mitochondrial DNA instability in cells lacking aconitase correlates with iron citrate toxicity.
Oxid Med Cell Longev. 2013;2013:493536. doi: 10.1155/2013/493536. Epub 2013 Aug 26.
6
Mgm101p is a novel component of the mitochondrial nucleoid that binds DNA and is required for the repair of oxidatively damaged mitochondrial DNA.
J Cell Biol. 1999 Apr 19;145(2):291-304. doi: 10.1083/jcb.145.2.291.
7
The role of Pif1p, a DNA helicase in Saccharomyces cerevisiae, in maintaining mitochondrial DNA.
Mitochondrion. 2007 May;7(3):211-22. doi: 10.1016/j.mito.2006.11.023. Epub 2006 Dec 9.
8
Biochemical and genetic characterization of Hmi1p, a yeast DNA helicase involved in the maintenance of mitochondrial DNA.
Yeast. 2005 Dec;22(16):1269-86. doi: 10.1002/yea.1313.
9
Association of the yeast DNA helicase Pif1p with mitochondrial membranes and mitochondrial DNA.
Eur J Cell Biol. 2010 Oct;89(10):742-7. doi: 10.1016/j.ejcb.2010.06.008. Epub 2010 Jul 23.
10
Differential involvement of the related DNA helicases Pif1p and Rrm3p in mtDNA point mutagenesis and stability.
Gene. 2005 Jul 18;354:86-92. doi: 10.1016/j.gene.2005.03.031.
引用本文的文献
1
Mitochondrial Aconitase and Its Contribution to the Pathogenesis of Neurodegenerative Diseases.
Int J Mol Sci. 2024 Sep 15;25(18):9950. doi: 10.3390/ijms25189950.
2
Kinetic and Regulatory Properties of Aconitate Hydratase as a Model-Indicator of Cell Redox State under pH Stress.
Int J Mol Sci. 2023 Apr 21;24(8):7670. doi: 10.3390/ijms24087670.
3
Genome-Wide Identification and Expression Profiling of Aconitase Gene Family Members Reveals Their Roles in Plant Development and Adaptation to Diverse Stress in L.
Plants (Basel). 2022 Dec 12;11(24):3475. doi: 10.3390/plants11243475.
4
Genetic screens reveal a central role for heme metabolism in artemisinin susceptibility.
Nat Commun. 2020 Sep 23;11(1):4813. doi: 10.1038/s41467-020-18624-0.
5
Mitochondrial HMG-Box Containing Proteins: From Biochemical Properties to the Roles in Human Diseases.
Biomolecules. 2020 Aug 16;10(8):1193. doi: 10.3390/biom10081193.
6
Metabolic Remodeling during Long-Lasting Cultivation of the Yeast on Oxidative and Fermentative Substrates.
Microorganisms. 2020 Jan 9;8(1):91. doi: 10.3390/microorganisms8010091.
7
Nitric Oxide Improves the Tolerance of Pleurotus ostreatus to Heat Stress by Inhibiting Mitochondrial Aconitase.
Appl Environ Microbiol. 2020 Feb 18;86(5). doi: 10.1128/AEM.02303-19.
8
A quantitative screen for metabolic enzyme structures reveals patterns of assembly across the yeast metabolic network.
Mol Biol Cell. 2019 Oct 1;30(21):2721-2736. doi: 10.1091/mbc.E19-04-0224. Epub 2019 Sep 4.
9
Exploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins From BCG Moreau and Pasteur by Comparative Proteomic.
Front Immunol. 2019 Apr 26;10:716. doi: 10.3389/fimmu.2019.00716. eCollection 2019.
10
Yeast aconitase mitochondrial import is modulated by interactions of its C and N terminal domains and Ssa1/2 (Hsp70).
Sci Rep. 2018 Apr 12;8(1):5903. doi: 10.1038/s41598-018-24068-w.
本文引用的文献
1
The role of Pif1p, a DNA helicase in Saccharomyces cerevisiae, in maintaining mitochondrial DNA.
Mitochondrion. 2007 May;7(3):211-22. doi: 10.1016/j.mito.2006.11.023. Epub 2006 Dec 9.
2
Structure of dual function iron regulatory protein 1 complexed with ferritin IRE-RNA.
Science. 2006 Dec 22;314(5807):1903-8. doi: 10.1126/science.1133116.
3
The role of iron regulatory proteins in mammalian iron homeostasis and disease.
Nat Chem Biol. 2006 Aug;2(8):406-14. doi: 10.1038/nchembio807.
4
Cotranscriptional processes and their influence on genome stability.
Genes Dev. 2006 Jul 15;20(14):1838-47. doi: 10.1101/gad.1438306.
5
Human mitochondrial DNA nucleoids are linked to protein folding machinery and metabolic enzymes at the mitochondrial inner membrane.
J Biol Chem. 2006 Sep 1;281(35):25791-802. doi: 10.1074/jbc.M604501200. Epub 2006 Jul 6.
6
Mitochondrial retrograde signaling.
Annu Rev Genet. 2006;40:159-85. doi: 10.1146/annurev.genet.40.110405.090613.
7
Crystal structure of human iron regulatory protein 1 as cytosolic aconitase.
Structure. 2006 Jan;14(1):129-39. doi: 10.1016/j.str.2005.09.009.
8
The organization and inheritance of the mitochondrial genome.
Nat Rev Genet. 2005 Nov;6(11):815-25. doi: 10.1038/nrg1708.
9
Structural and functional characterizations of mung bean mitochondrial nucleoids.
Nucleic Acids Res. 2005 Aug 22;33(15):4725-39. doi: 10.1093/nar/gki783. Print 2005.
10
The conserved Mec1/Rad53 nuclear checkpoint pathway regulates mitochondrial DNA copy number in Saccharomyces cerevisiae.
Mol Biol Cell. 2005 Jun;16(6):3010-8. doi: 10.1091/mbc.e05-01-0053. Epub 2005 Apr 13.
Zotero 插件