Marcó J, Jiménez-Vargas J
Rev Esp Fisiol. 1979 Mar;35(1):75-83.
The effect of copulating on reflex ovulation was studied in rat. The effect of PCPA (300 mg/kg, i.p.) on ovulation and reproduction was compared by evaluating number of oocytes in tubes, histologic features of ovaries, vaginal cycle, insemination, fertilization and number of embryos per rat. PCPA, administered on 9th and 16th h of the estrus phase, totally inhibits ovulation, stimulates reproductive behaviour and prolongs the estrogenic phase. When the animals are kept in copulatory conditions for 16 or 46 hours, the inhibition induced in ovulation disappears to the extent that 60% of the rats become pregnant though the number of embryos is under that of the control group. The farther the treatment with PCPA within the same cycle in the ovulatory period, the greater the inhibitory effect on ovulation is and the lesser the neutralizing effect produced by reflexes related to copulation. Administration of PCPA at the 16 hour of the diestrus causes a greater increase in the average number of embryos-as compared to administration at the 9 hour. In periods longer than 48 hours before ovulation, the inhibition brought about by PCPA is not suppressed by copulatory conditions kept for 16 or 24 hours and is only neutralized if they are kept during a complete cycle. Those treated with PCPA in the diestrus phase and maintained in copulatory conditions for 46 hours, present a higher average of embryos than those maintained in similar conditions for 16 hours.
研究了交配对大鼠反射性排卵的影响。通过评估输卵管中的卵母细胞数量、卵巢的组织学特征、阴道周期、授精、受精以及每只大鼠的胚胎数量，比较了对氯苯丙氨酸（PCPA，300毫克/千克，腹腔注射）对排卵和生殖的影响。在发情期的第9小时和第16小时给予PCPA，可完全抑制排卵，刺激生殖行为并延长雌激素期。当动物处于交配状态16或46小时时，排卵抑制作用消失，60%的大鼠怀孕，尽管胚胎数量低于对照组。在排卵期同一周期内给予PCPA的时间越晚，对排卵的抑制作用越强，与交配相关的反射产生的中和作用越小。与在第9小时给药相比，在动情后期第16小时给予PCPA可使胚胎平均数量有更大增加。在排卵前超过48小时的时间段内，PCPA引起的抑制作用不会被保持16或24小时的交配状态所抑制，只有在整个周期内保持交配状态时才会被中和。在动情后期用PCPA处理并在交配状态下维持46小时的大鼠，其胚胎平均数量高于在类似条件下维持16小时的大鼠。
