Thomas J, Thomas F, Hoffmann S, Johns C, Lee H M
Surgery. 1979 Aug;86(2):266-74.
In this study 66 renal transplant recipients were studied by in vitro immunologic monitoring assays in an attempt to delineate some immune reactivity parameters which migh explain the successful long-term survival of HLA incompatible allografts. Ninety percent of the recipients were found to be responsive to their specific donors in one-way mixed lymphocyte culture assays, indicating the presence of antigen reactive proliferating T cells. In contrast, studies of the in vitro generation of cytotoxic T cells showed that 73% of the long-term recipients demonstrated a marked hyporesponsiveness to the donor in cell-mediated lympholysis (CML) assays. Longitudinal studies indicated that specific CML hyporesponsiveness to the donor developed after transplantion. In the majority of recipients, CML hyporesponsiveness could be related to a suppressor cell phenomenon, since recipient mononuculear cells specifically suppressed the CML reactivity of third-party cells to the donor. Recipient CML hyporesponsiveness to the donor could be abrogated by removal of recipient adherent cells, most of which were monocytes or macrophages. The suppressor cell effect on CML is postulated to be related to macrophages which are either acting independently or under the regulation of suppressor T cells. These studies are among the first to suggest that suppressor cell regulation of relevant effector T cell activity may be important in the facilitation of successful human renal transplantation.
在本研究中，对66名肾移植受者进行了体外免疫监测分析，以试图描绘一些免疫反应参数，这些参数可能解释HLA不相容同种异体移植物的长期成功存活。在单向混合淋巴细胞培养分析中，发现90%的受者对其特定供体有反应，表明存在抗原反应性增殖T细胞。相比之下，对细胞毒性T细胞体外生成的研究表明，73%的长期受者在细胞介导的淋巴细胞溶解（CML）分析中对供体表现出明显的低反应性。纵向研究表明，移植后出现了对供体的特异性CML低反应性。在大多数受者中，CML低反应性可能与抑制细胞现象有关，因为受者单核细胞特异性抑制了第三方细胞对供体的CML反应性。去除受者贴壁细胞（其中大多数是单核细胞或巨噬细胞）可消除受者对供体的CML低反应性。推测抑制细胞对CML的作用与巨噬细胞有关，巨噬细胞要么独立发挥作用，要么在抑制性T细胞的调节下发挥作用。这些研究首次表明，相关效应T细胞活性的抑制细胞调节可能对促进人类肾移植成功很重要。
