通过臭氧诱导羧基甜菜碱单体接枝聚合在硅橡胶表面实现血小板黏附和蛋白质吸附。

Platelet adhesion and protein adsorption on silicone rubber surface by ozone-induced grafted polymerization with carboxybetaine monomer.

作者信息

Zhou Jun, Yuan Jiang, Zang Xiaopeng, Shen Jian, Lin Sicong

机构信息

College of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.

出版信息

Colloids Surf B Biointerfaces. 2005 Mar 10;41(1):55-62. doi: 10.1016/j.colsurfb.2004.11.006.

DOI:10.1016/j.colsurfb.2004.11.006

PMID:15698757

Abstract

Platelet adhesion and protein adsorption on the silicone rubber film grafted with N,N'-dimethyl-N-methacryloyloxyethyl-N-(2-carboxyethyl) ammonium (DMMCA) was studied. The grafting was carried out by means of ozone-induced method and was confirmed by ATR-FTIR and XPS investigations. The grafted films possessed relatively hydrophilic surface revealed by contact angle measurement. The blood compatibility of the grafted film was evaluated in vitro by platelet adhesion in platelet-rich plasma (PRP) and protein absorption in bovine fibrinogen (BFG) using silicone film as the reference. No substantial platelet adhesion was observed for the grafted films incubated in PRP for 60 and 180 min. The protein absorption was also significantly reduced after incubated in bovine fibrinogen for 60 min. Both the results indicated that the blood compatibility of silicone rubber was greatly improved by ozone-induced grafting of carboxybetaine zwitterionic polymer onto its surface.

摘要

研究了接枝N，N'-二甲基-N-甲基丙烯酰氧基乙基-N-（2-羧乙基）铵（DMMCA）的硅橡胶膜上的血小板粘附和蛋白质吸附。接枝通过臭氧诱导法进行，并通过ATR-FTIR和XPS研究得到证实。通过接触角测量表明，接枝膜具有相对亲水的表面。以硅膜为参照，通过富血小板血浆（PRP）中的血小板粘附和牛纤维蛋白原（BFG）中的蛋白质吸收，在体外评估接枝膜的血液相容性。在PRP中孵育60分钟和180分钟后，接枝膜未观察到大量血小板粘附。在牛纤维蛋白原中孵育60分钟后，蛋白质吸收也显著降低。这两个结果表明，通过臭氧诱导在硅橡胶表面接枝羧基甜菜碱两性离子聚合物，其血液相容性得到了极大改善。