School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, People's Republic of China.
Mater Sci Eng C Mater Biol Appl. 2013 Oct;33(7):3865-74. doi: 10.1016/j.msec.2013.05.025. Epub 2013 May 17.
A facile approach to modify silicone rubber (SR) membrane for improving the blood compatibility was investigated. The hydrophobic SR surface was firstly activated by air plasma, after which an initiator was immobilized on the activated surface for atom transfer radical polymerization (ATRP). Three zwitterionic polymers were then grafted from SR membrane via surface-initiated atom transfer radical polymerization (SI-ATRP). The surface composition, wettability, and morphology of the membranes before and after modification were characterized by X-ray photoelectron spectroscopy (XPS), static water contact angle (WCA) measurement, and atomic force microscopy (AFM). Results showed that zwitterionic polymers were successfully grafted from SR surfaces, which remarkably improved the wettability of the SR surface. The blood compatibility of the membranes was evaluated by protein adsorption and platelet adhesion tests in vitro. As observed, all the zwitterionic polymer modified surfaces have improved resistance to nonspecific protein adsorption and have excellent resistance to platelet adhesion, showing significantly improved blood compatibility. This work should inspire many creative uses of SR based materials for biomedical applications such as vessel, catheter, and microfluidics.
本研究旨在提出一种简便的方法，通过对硅橡胶（SR）膜进行改性来提高其血液相容性。首先，通过空气等离子体对疏水性的 SR 表面进行活化，然后将引发剂固定在活化表面上，用于原子转移自由基聚合（ATRP）。接着，通过表面引发原子转移自由基聚合（SI-ATRP），将三种两性离子聚合物从 SR 膜上接枝。通过 X 射线光电子能谱（XPS）、静态水接触角（WCA）测量和原子力显微镜（AFM）对膜改性前后的表面组成、润湿性和形貌进行了表征。结果表明，两性离子聚合物成功地从 SR 表面接枝，显著提高了 SR 表面的润湿性。通过体外蛋白质吸附和血小板黏附试验评价了膜的血液相容性。结果表明，所有接枝有两性离子聚合物的表面均能显著提高对非特异性蛋白质吸附的抵抗力，且对血小板黏附具有极好的抵抗力，显示出显著改善的血液相容性。这项工作应该会激发许多基于 SR 的材料在血管、导管和微流控等生物医学应用中的创造性用途。
