Zhao F X, Liu S Y, Si J Y
Department of Microbiology, Datong Medical College.
Zhonghua Zhong Liu Za Zhi. 1994 Mar;16(2):102-5.
Biopsy materials of cervical carcinoma including 20 cervical adenocarcinomas and 20 squamous cell carcinomas were collected. A rapid method for determining HPV type was developed, based on DdeI restriction enzymes analysis within the L1 region of HPV, amplified by PCR using consensus primers. The results indicated that HPV type 16 was detected more often in squamous cell carcinomas than in adenocarcinomas (80% vs 15%, P < 0.001), conversely, HPV type 18 was detected significantly more often in adenocarcinoma tissues (45% vs 5%, P < 0.001). These differences may reflect the presence of different virus receptors in cancer cells with different morphologic potential, or, they may indicate that the specific HPV infection actually plays a direct role in the process of carcinogenesis.
收集了包括20例宫颈腺癌和20例鳞状细胞癌的宫颈癌活检材料。基于对人乳头瘤病毒（HPV）L1区域内DdeI限制性酶切分析，开发了一种快速确定HPV类型的方法，该区域通过使用共有引物的聚合酶链反应（PCR）进行扩增。结果表明，HPV 16型在鳞状细胞癌中的检出率高于腺癌（80%对15%，P<0.001），相反，HPV 18型在腺癌组织中的检出率显著更高（45%对5%，P<0.001）。这些差异可能反映了具有不同形态学潜能的癌细胞中存在不同的病毒受体，或者，它们可能表明特定的HPV感染实际上在致癌过程中起直接作用。
