Fulzele Devanand P, Satdive Ramesh K
Plant Biotechnology and Secondary Products Section, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India.
J Chromatogr A. 2005 Jan 21;1063(1-2):9-13. doi: 10.1016/j.chroma.2004.11.020.
Extraction methods using stirring extraction, Soxhlet extraction, ultrasonic extraction and microwave-assisted extraction (MAE) were evaluated for the percentage extraction of camptothecin (CPT) and 9-methoxycamptothecin (9-Me-CPT) from Nothapodytes foetida. The extracts were analyzed by high performance liquid chromatography (HPLC). Methanol (90%, v/v) extracted high percentage extraction of CPT and 9-Me-CPT compared to ethanol (90%, v/v). The results shows that the percentage extraction of CPT and 9-Me-CPT from N. foetida by MAE was more efficient in short time followed by Soxhlet extraction, ultrasonic and stirring extraction methods. Maximum percentage extraction of CPT (2.67%, w/w) was obtained by MAE technique. MAE has need of 3 min, whereas ultrasonic extraction, Soxhlet extraction and stirring extraction techniques require 30, 120 and 30 min, respectively to leach higher percentage extraction of CPT and 9-Me-CPT. The times taken by the microwave extraction process was 40 times less than the Soxhlet extraction for percentage extraction of alkaloids. The present results show that the extraction efficiency and considerable saving of time by MAE was more competent than the other extraction techniques.
对采用搅拌萃取、索氏萃取、超声萃取和微波辅助萃取（MAE）从印度蛇根木中提取喜树碱（CPT）和9-甲氧基喜树碱（9-Me-CPT）的方法进行了评估。提取物通过高效液相色谱（HPLC）进行分析。与乙醇（90%，v/v）相比，甲醇（90%，v/v）对CPT和9-Me-CPT的提取率更高。结果表明，MAE从印度蛇根木中提取CPT和9-Me-CPT的效率在短时间内更高，其次是索氏萃取、超声萃取和搅拌萃取方法。MAE技术获得了CPT的最大提取率（2.67%，w/w）。MAE需要3分钟，而超声萃取、索氏萃取和搅拌萃取技术分别需要30、120和30分钟才能浸出更高比例的CPT和9-Me-CPT。微波萃取过程提取生物碱的时间比索氏萃取少40倍。目前的结果表明，MAE的提取效率和显著的时间节省比其他萃取技术更具优势。
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