Namdeo A G, Sharma A
Department of Pharmacognosy, Poona College of Pharmacy, Bharati Vidyapeeth University, Pune, Maharashtra - 411038, India.
Asian Pac J Trop Biomed. 2012 May;2(5):389-93. doi: 10.1016/S2221-1691(12)60062-8.
To investigate the content of topoisomerase I-DNA inhibitor alkaloid camptothecin (CPT) from various parts of Nothapodytes foetida (N. foetida) collected from the month of October to February.
The content of CPT was quantified in the methanolic extract of various parts of N. foetida using high performance liquid chromatography (HPLC). Quantification was performed with the regression analysis and the method was validated as per ICH guidelines.
The results revealed that maximum concentrations of camptothecin were found in root (2.62%) collected in the month of February followed by fruits (January, 1.22%), stem (January, 0.81%) and leaves (February, 0.70%). Roots were found to have 3-fold higher concentration of CPT than the leaves and stem, while the fruits showed 2-fold higher concentration. Maximum concentration of camptothecin in fruits was observed in month of January, when they were not fully mature, which was 2-fold higher than that of young and fully mature fruits.
These findings indicate that the synthesis of CPT differs in different parts of N. foetida and the content varies periodically.
研究10月至2月采集的臭椿不同部位中拓扑异构酶I-DNA抑制剂生物碱喜树碱（CPT）的含量。
采用高效液相色谱法（HPLC）对臭椿不同部位的甲醇提取物中的CPT含量进行定量分析。通过回归分析进行定量，并按照国际人用药品注册技术协调会（ICH）指南对该方法进行验证。
结果显示，2月采集的根部中喜树碱浓度最高（2.62%），其次是果实（1月，1.22%）、茎（1月，0.81%）和叶（2月，0.70%）。发现根部的CPT浓度比叶和茎高3倍，而果实的浓度则高2倍。1月果实未完全成熟时，喜树碱浓度最高，比幼果和完全成熟果实高2倍。
这些研究结果表明，臭椿不同部位CPT的合成存在差异，且含量呈周期性变化。
