Sharma Kishmita, Satdive Ramesh K, Singh Sudhir
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, 400085 India.
Homi Bhabha National Institute, Anushaktinagar, Mumbai, 400094 India.
3 Biotech. 2024 Jun;14(6):153. doi: 10.1007/s13205-024-03999-4. Epub 2024 May 11.
Genus has recently emerged as one of the promising sources of Camptothecin (CPT), an antitumour monoterpene indole alkaloid. It possesses CPT in its every part and has a relatively short life span. To determine whether differentiation plays any role in the synthesis and/or accumulation of CPT, the concentration of CPT was analyzed across various tissues of var. obtained through both direct as well as indirect modes of regeneration. The results revealed that the plants obtained from both types of regeneration showed similar levels of CPT. It was also observed that with differentiation, the accumulation of CPT increases, as the callus, being an undifferentiated mass of cells, had only traces of CPT. In contrast, the completely differentiated in-vitro plant obtained from it showed a significantly higher percentage of CPT in shoots (0.22% dry weight) and roots (0.247% dw). The CPT when analyzed after hardening, varied among different organs of the plant. It was also observed that the inflorescence accumulated the highest concentration of CPT (0.348% dw) once the flowering began, accompanied by a decrease in remaining organs. This decrease may result from CPT being mobilized to the inflorescence as a chemical defense mechanism. These findings allowed us to determine the ideal plant harvesting age for CPT extraction. The findings could be used to decide the right stage of plant harvest, which is just before the onset of blooming.
The online version contains supplementary material available at 10.1007/s13205-024-03999-4.
[植物属名]最近已成为喜树碱（CPT）的有前景的来源之一，喜树碱是一种抗肿瘤单萜吲哚生物碱。它的各个部分都含有喜树碱，且寿命相对较短。为了确定分化是否在喜树碱的合成和/或积累中起作用，分析了通过直接和间接再生方式获得的[植物品种名]不同组织中喜树碱的浓度。结果表明，从两种再生类型获得的植株喜树碱水平相似。还观察到随着分化，喜树碱的积累增加，因为愈伤组织作为未分化的细胞团，仅含有痕量的喜树碱。相比之下，从愈伤组织获得的完全分化的离体植株在茎（干重的0.22%）和根（干重的0.247%）中喜树碱的百分比显著更高。硬化后分析的喜树碱在植物的不同器官中有所不同。还观察到一旦开花开始，花序积累的喜树碱浓度最高（干重的0.348%），同时其余器官中的喜树碱含量下降。这种下降可能是由于喜树碱作为一种化学防御机制被转运到花序中。这些发现使我们能够确定喜树碱提取的理想植物收获年龄。这些发现可用于确定植物收获的正确阶段，即就在开花开始之前。
在线版本包含可在10.1007/s13205-024-03999-4获取的补充材料。
