Dion Anna, Berno Bob, Hall Gordon, Filiaggi Mark Joseph
School of Biomedical Engineering, Dalhousie University, Halifax, Nova Scotia, Canada B3H 3J5.
Biomaterials. 2005 Jul;26(21):4486-94. doi: 10.1016/j.biomaterials.2004.11.010.
Calcium polyphosphate antibiotic delivery matrices were prepared using a unique processing technique involving the exposure of calcium polyphosphate pastes to high humidity for 0, 5, 24 or 48 h to induce gelling. Subsequently, samples were dried for a minimum of 24 h. The mild conditions associated with matrix fabrication readily allowed for vancomycin incorporation within an environment that did not disrupt antibiotic activity. While reproducible from a processing standpoint, the gelling and drying process did contribute to a decrease in matrix tensile strength and the formation of significant pores near the surface of the matrices. Generally, the core of the gelled matrices appeared to be denser than their non-gelled counterparts. The degree of phosphate chain lysis during the gelling and drying stages was quantified using solution 31P nuclear magnetic resonance (NMR) spectroscopy. Both NMR and Raman spectroscopy indicated that the presence of vancomycin did not appreciably alter the matrix formation process. The ability to incorporate clinically relevant levels of antibiotic within this degradable bone substitute matrix suggests the potential of this approach for creating a localized antibiotic delivery system to treat osteomyelitis infections.
使用一种独特的加工技术制备了聚磷酸钙抗生素递送基质，该技术包括将聚磷酸钙糊剂暴露于高湿度环境中0、5、24或48小时以诱导凝胶化。随后，将样品干燥至少24小时。与基质制造相关的温和条件使得万古霉素能够在不破坏抗生素活性的环境中轻松掺入。虽然从加工角度来看具有可重复性，但凝胶化和干燥过程确实导致了基质拉伸强度的降低以及基质表面附近形成大量孔隙。一般来说，凝胶化基质的核心似乎比未凝胶化的对应物更致密。使用溶液31P核磁共振（NMR）光谱对凝胶化和干燥阶段的磷酸链裂解程度进行了量化。NMR和拉曼光谱均表明万古霉素的存在并未明显改变基质形成过程。在这种可降解骨替代基质中掺入临床相关水平抗生素的能力表明了这种方法用于创建局部抗生素递送系统以治疗骨髓炎感染的潜力。
