Englert C, Angele P, Fierlbeck J, Dendorfer S, Schubert T, Müller R, Lienhard S, Zellner J, Nerlich M, Neumann C
Abteilung Unfallchirurgie, Klinikum der Universität, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany.
Unfallchirurg. 2007 May;110(5):408-13. doi: 10.1007/s00113-007-1229-3.
A new bone substitute, consisting of hydroxylapatite and calcium sulphate, was prepared in two formulations and analysed for its mechanical strength and antibiotic elution.
The bone substitute PerOssal has osteoconductive and degradable properties. The material has a built-in capillary structure, which results in an immediate fluid uptake. Antibiotics absorbed to the bone substitute resulted in a prolonged release rate. Mechanical strength was investigated by an unconfined compression test up to failure under both wet and dry conditions for both formulations of the bone substitute. Antibiotic release was analysed microbiologically for two antibiotics, vancomycin and gentamicin, over an elution period of 10 days using the agar diffusion method.
The drug release analysis resulted in a prolonged release rate of both antibiotics over 10 days. In vitro the amount of gentamicin and vancomycin eluted at day 10. From one pellet still exceeded the minimal inhibitory concentration of most aetiologically important pathogens. Formulation two of the present bone substitute is significantly harder in both wet and dry conditions when compared to formulation one. Both formulations lose strength in the wet condition relative to their performance in the dry condition. However, formulation two is as hard under wet conditions as formulation one is when dry.
PerOssal is a suitable new degradable osteoconductive bone substitute that can be loaded with antibiotic solutions, which are released in effective doses over 10 days. The mechanical strength of PerOssal is sufficient to support cancellous bone defects in non-weight-bearing areas or in combination with osteosynthesis.
制备了一种由羟基磷灰石和硫酸钙组成的新型骨替代物，有两种配方，并对其机械强度和抗生素洗脱情况进行了分析。
骨替代物PerOssal具有骨传导性和可降解性。该材料具有内置的毛细管结构，可立即吸收液体。吸附在骨替代物上的抗生素导致释放速率延长。通过无侧限压缩试验研究了两种配方的骨替代物在湿态和干态下直至破坏时的机械强度。使用琼脂扩散法，在10天的洗脱期内，对万古霉素和庆大霉素这两种抗生素进行微生物学分析，以分析抗生素释放情况。
药物释放分析表明，两种抗生素在10天内的释放速率都有所延长。在体外，第10天时从一个小球中洗脱的庆大霉素和万古霉素量仍超过大多数重要病原菌的最低抑菌浓度。与配方一相比，本骨替代物的配方二在湿态和干态下都明显更硬。相对于其在干态下的性能，两种配方在湿态下都会失去强度。然而，配方二在湿态下的硬度与配方一在干态下的硬度相同。
PerOssal是一种合适的新型可降解骨传导性骨替代物，可加载抗生素溶液，这些溶液在10天内以有效剂量释放。PerOssal的机械强度足以支撑非负重区域的松质骨缺损或与骨固定联合使用。
