“组学”时代的蛋白质定位研究。

Protein localization studies in the age of 'Omics'.

作者信息

O'Rourke Nancy A, Meyer Tobias, Chandy Grischa

机构信息

Alliance for Cell Signaling, Microscopy Lab, Stanford University School of Medicine, 975 California Ave, Palo Alto, CA 94304, USA.

出版信息

Curr Opin Chem Biol. 2005 Feb;9(1):82-7. doi: 10.1016/j.cbpa.2004.12.002.

DOI:10.1016/j.cbpa.2004.12.002

PMID:15701458

Abstract

As thousands of new genes are identified in genomics efforts, the rush is on to learn something about the functional roles of the proteins encoded by those genes. Clues to protein functions, activation states and protein-protein interactions have been revealed in focused studies of protein localization. With technical breakthroughs such as GFP protein tagging and recombinase cloning systems, large-scale screens of protein localization are now being undertaken.

摘要

随着基因组学研究中数千个新基因被识别出来，人们急于了解这些基因所编码蛋白质的功能作用。在对蛋白质定位的重点研究中，已经揭示了蛋白质功能、激活状态及蛋白质 - 蛋白质相互作用的线索。借助诸如绿色荧光蛋白（GFP）标记和重组酶克隆系统等技术突破，目前正在进行大规模的蛋白质定位筛选。