青霉霉菌毒素桔霉素、环匹阿尼酸、赭曲霉毒素A、展青霉素、青霉酸和罗克福汀C对猪淋巴细胞体外增殖的影响。

The effects of the Penicillium mycotoxins citrinin, cyclopiazonic acid, ochratoxin A, patulin, penicillic acid, and roquefortine C on in vitro proliferation of porcine lymphocytes.

作者信息

Keblys Modestas, Bernhoft Aksel, Höfer Constance C, Morrison Ellen, Larsen Hans Jørgen S, Flåøyen Arne

机构信息

National Veterinary Institute, P.O. Box 8156 Dep., N-0033, Oslo, Norway.

出版信息

Mycopathologia. 2004 Oct;158(3):317-24. doi: 10.1007/s11046-005-5523-8.

DOI:10.1007/s11046-005-5523-8

PMID:15702270

Abstract

The in vitro effect of each of the Penicillium mycotoxins citrinin (CIT), cyclopiazonic acid (CPA), ochratoxin A (OTA), patulin (PAT), penicillic acid (PIA) and roquefortine C (RQC) on mitogen induced lymphocyte proliferation was determined using purified lymphocytes from 6 piglets. Dose response curves for each mycotoxin were generated and the concentrations producing 50% inhibition of cell proliferation (IC(50)) were estimated. OTA and PAT were the most potent toxins with IC(50) of 1.3 and 1.2 micromol/l, respectively (0.52 and 0.18 mg/l, respectively). Based on molar concentrations, OTA was 15, 30, 40, and 65 times more potent as an inhibitor than PIA, CIT, CPA and RQC, respectively.

摘要

使用6头仔猪的纯化淋巴细胞，测定了青霉属霉菌毒素桔青霉素（CIT）、环匹阿尼酸（CPA）、赭曲霉毒素A（OTA）、展青霉素（PAT）、青霉酸（PIA）和罗克福汀C（RQC）对有丝分裂原诱导的淋巴细胞增殖的体外作用。生成了每种霉菌毒素的剂量反应曲线，并估计了产生50%细胞增殖抑制（IC50）的浓度。OTA和PAT是最有效的毒素，IC50分别为1.3和1.2微摩尔/升（分别为0.52和0.18毫克/升）。基于摩尔浓度，OTA作为抑制剂的效力分别比PIA、CIT、CPA和RQC高15、30、40和65倍。