DNA对铜（II）催化过氧化氢分解产生的化学发光的增强作用。

Enhancing effect of DNA on chemiluminescence from the decomposition of hydrogen peroxide catalyzed by copper(II).

作者信息

Liu Meilin, Li Baoxin, Zhang Zhujun, Lin Jin-Ming

机构信息

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, 100085 Beijing, P.R. China.

出版信息

Anal Bioanal Chem. 2005 Feb;381(4):828-32. doi: 10.1007/s00216-004-2922-5. Epub 2005 Feb 9.

DOI:10.1007/s00216-004-2922-5

PMID:15702312

Abstract

In the absence of any special luminescence reagent, emission of weak chemiluminescence has been observed during the decomposition of hydrogen peroxide catalyzed by copper(II) in basic aqueous solution. The intensity of the chemiluminescence was greatly enhanced by addition of DNA and was strongly dependent on DNA concentration. Based on these phenomena, a flow-injection chemiluminescence method was established for determination of DNA. The chemiluminescence intensity was linear with DNA concentration in the range 2 x 10(-7)-1 x 10(-5) g L(-1) and the detection limit was 4.1 x 10(-8) g L(-1) (S/N=3). The relative standard deviation was less than 3.0% for 4 x 10(-7) g L(-1) DNA (n=11). The proposed method was satisfactorily applied for determination of DNA in synthetic samples. The possible mechanism of the CL reaction is discussed.

摘要

在没有任何特殊发光试剂的情况下，已观察到在碱性水溶液中铜（II）催化过氧化氢分解过程中会发出微弱的化学发光。加入DNA后，化学发光强度大大增强，且强烈依赖于DNA浓度。基于这些现象，建立了一种流动注射化学发光法测定DNA。化学发光强度与DNA浓度在2×10⁻⁷ - 1×10⁻⁵ g L⁻¹范围内呈线性关系，检测限为4.1×10⁻⁸ g L⁻¹（S/N = 3）。对于4×10⁻⁷ g L⁻¹的DNA，相对标准偏差小于3.0%（n = 11）。该方法成功应用于合成样品中DNA的测定。讨论了化学发光反应的可能机制。