Khataee Alireza, Iranifam Mortaza, Fathinia Mehrangiz, Nikravesh Mina
Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Department of Chemistry, Faculty of Science, University of Maragheh, Maragheh, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Jan 5;134:210-7. doi: 10.1016/j.saa.2014.06.125. Epub 2014 Jun 28.
In this paper, a rapid and sensitive flow-injection chemiluminescence (flow-CL) system was developed for the determination of cloxacillin sodium in environmental water samples and pharmaceutical preparations. The method was based on the enhancement effect of cloxacillin sodium on the CL reaction of luminal-H₂O₂-CuO nanosheets (NSs) in alkaline medium. The CuO nanosheets were synthesized using a green sonochemical method. The physical properties of the synthesized CuO nanosheets were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM) analyses. The influences of various experimental factors such as H₂O₂, NaOH, luminol and CuO nanosheets concentrations were investigated. Under the optimum conditions, the enhanced CL intensity was linearly related to the concentration of cloxacillin sodium in the range of the 0.05-30.00 mg L(-1) with a correlation coefficient of 0.995. The corresponding detection limit (3σ) was calculated to be 0.026 mg L(-1). The relative standard deviation (RSD) of the developed method was 2.21% with 11 repeated measurements of 4.00 mg L(-1) cloxacillin sodium. Also, a total analysis time per sample was 30 s which confirmed the rapidity of the proposed method. The analytical applicability of the proposed CL system was assessed by determining cloxacillin sodium in spiked environmental water samples and pharmaceutical preparation. Furthermore, the possible mechanism of CL reaction was discussed.
本文建立了一种快速灵敏的流动注射化学发光（流动 - CL）体系，用于测定环境水样和药物制剂中的氯唑西林钠。该方法基于碱性介质中氯唑西林钠对鲁米诺 - H₂O₂ - 氧化铜纳米片（NSs）化学发光反应的增强作用。采用绿色超声化学法合成了氧化铜纳米片。通过X射线衍射（XRD）和扫描电子显微镜（SEM）分析对合成的氧化铜纳米片的物理性质进行了表征。研究了H₂O₂、NaOH、鲁米诺和氧化铜纳米片浓度等各种实验因素的影响。在最佳条件下，增强的化学发光强度与氯唑西林钠浓度在0.05 - 30.00 mg L⁻¹范围内呈线性关系，相关系数为0.995。计算得到相应的检测限（3σ）为0.026 mg L⁻¹。对4.00 mg L⁻¹氯唑西林钠进行11次重复测量，该方法的相对标准偏差（RSD）为2.21%。而且，每个样品的总分析时间为30 s，证实了该方法的快速性。通过测定加标环境水样和药物制剂中的氯唑西林钠，评估了所提出的化学发光体系的分析适用性。此外，还讨论了化学发光反应的可能机理。
