Iranifam Mortaza, Kharameh Merhnaz Khabbaz
Department of Chemistry, Faculty of Science, University of Maragheh, Maragheh, East Azerbaijan, Iran.
Luminescence. 2014 Sep;29(6):679-83. doi: 10.1002/bio.2606. Epub 2013 Nov 19.
A simple and sensitive chemiluminescence (CL) method has been developed for the determination of ampicillin sodium at submicromolar levels. The method is based on the inhibitory effect of ampicillin sodium on the cupric oxide nanoparticles (CuO NPs)-luminol-H2 O2 CL reaction. Experimental parameters affecting CL inhibition including concentrations of CuO NPs, luminol, H2 O2 and NaOH were optimized. Under optimum conditions, the calibration plot was linear in the analyte concentration range 4.0 × 10(-7) -4.0 × 10(-6) mol/L. The limit of detection was 2.6 × 10(-7) mol/L and the relative standard deviation (RSD) for six replicate determinations of 1 × 10(-6) mol/L ampicillin sodium was 4.71%. Also, X-ray diffraction (XRD) and transmission electron microscopy (TEM) analysis were employed to characterize the CuO NPs. The utility of the proposed method was demonstrated by determining ampicillin sodium in pharmaceutical preparation.
已开发出一种简单且灵敏的化学发光（CL）方法，用于测定亚微摩尔水平的氨苄西林钠。该方法基于氨苄西林钠对氧化铜纳米颗粒（CuO NPs）-鲁米诺-H₂O₂ CL反应的抑制作用。优化了影响CL抑制的实验参数，包括CuO NPs、鲁米诺、H₂O₂和NaOH的浓度。在最佳条件下，校准曲线在分析物浓度范围4.0×10⁻⁷ - 4.0×10⁻⁶ mol/L内呈线性。检测限为2.6×10⁻⁷ mol/L，对1×10⁻⁶ mol/L氨苄西林钠进行六次重复测定的相对标准偏差（RSD）为4.71%。此外，采用X射线衍射（XRD）和透射电子显微镜（TEM）分析对CuO NPs进行表征。通过测定药物制剂中的氨苄西林钠证明了该方法的实用性。
