Department of Biological, Geological and Environmental Sciences, University of Bologna, 40126 Bologna, Italy.
Int J Mol Sci. 2023 Jun 30;24(13):10953. doi: 10.3390/ijms241310953.
In vertebrates, neurotrophins and their receptors play a fundamental role in the central and peripheral nervous systems. Several studies reported that each neurotrophin/receptor signalling pathway can perform various functions during axon development, neuronal growth, and plasticity. Previous investigations in some fish species have identified neurotrophins and their receptors in the spinal cord under physiological conditions and after injuries, highlighting their potential role during regeneration. In our study, for the first time, we used an excellent animal model, the zebrafish (), to compare the mRNA localization patterns of neurotrophins and receptors in the spinal cord. We quantified the levels of mRNA using qPCR, and identified the transcription pattern of each neurotrophin/receptor pathway via in situ hybridization. Our data show that are the most transcribed members in the adult zebrafish spinal cord.
在脊椎动物中，神经营养因子及其受体在中枢和外周神经系统中起着至关重要的作用。多项研究表明，每种神经营养因子/受体信号通路在轴突发育、神经元生长和可塑性方面都能发挥多种功能。以前在一些鱼类物种中的研究已经在生理条件和损伤后确定了脊髓中的神经营养因子及其受体，突出了它们在再生过程中的潜在作用。在我们的研究中，我们首次使用了一种优秀的动物模型——斑马鱼（），来比较神经营养因子和受体在脊髓中的 mRNA 定位模式。我们使用 qPCR 来量化 mRNA 的水平，并通过原位杂交来确定每个神经营养因子/受体途径的转录模式。我们的数据表明， 在成年斑马鱼脊髓中是转录水平最高的成员。
