[高效液相色谱法测定秋季采收的茵陈蒿中滨蓟黄醇的含量]
[Determination of cirsilineol in Herba Artemisiae scopariae collected in autumn by HPLC].
作者信息
Zhang Qi-wei, Zhang Yong-xin, Zhang Ying, Liang Guo-gang
机构信息
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2002 Jan;27(1):23-5.
OBJECTIVE
To establish a quantitative method for determination of cirsilineol in Herba Artemisiae Scopariae collected in Autumn.
METHOD
Sample was extracted with methanol, and cleaned up with polyamide column. ODS column was used with methanol-acetonitrile-0.5% acetic acid solution (24:19:57) as mobile phase. Detection wavelength was 347 nm.
RESULT
Cirsilineol in sample solution was well separated. Linearity of cirsilineol was good (r = 0.9998) in range of 0.1-0.8 microgram. The average recovery was 101.3%, RSD of repeatability was 3.04%.
CONCLUSION
This method can be used for quality control of Herba Artemisiae Scopariae collected in autumn and its preparations.
目的
建立秋季采收的茵陈中滨蓟黄醇的定量测定方法。
方法
样品用甲醇提取，经聚酰胺柱净化。采用ODS柱，以甲醇-乙腈-0.5%醋酸溶液（24:19:57）为流动相。检测波长为347nm。
结果
样品溶液中滨蓟黄醇分离良好。滨蓟黄醇在0.1 - 0.8微克范围内线性良好（r = 0.9998）。平均回收率为101.3%，重复性RSD为3.04%。
结论
该方法可用于秋季采收的茵陈及其制剂的质量控制。
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