高效液相色谱法测定石香薷中香芹酚和百里香酚的含量
[Determination of carvacrol and thymol in Mosla chinensis by HPLC].
作者信息
Ji Li, Wang Fang, Liu Yuan-yan, Tong Yan, Li Xian-duan, Feng Xue-feng, Huang Lu-qi, Zhou Guo-ping
机构信息
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2004 Nov;29(11):1030-2.
OBJECTIVE
To establish a quantitative method of determination of carvacrol and thymol in Mosla chinensis.
METHOD
The sample was extracted with 95% ethanol, ODS column was used with methanol-water-acetic acid (60:40:2) as mobile phase. The detection wavelength was set at 274 nm.
RESULT
The linearities of carvacrol and thymol were respectively in the range of 0.23-2.15 microg (r = 0.9999) and 0.39-2.36 microg (r = 0.9999); the average recoveries were 99.9% (RSD 1.4%) and 98.6% (RSD 1.3%); the RSD of repeatability were 1.1% and 1.6%.
CONCLUSION
The method is reliable, and can be used for quality control of M. chinensis.
目的
建立一种测定石香薷中香芹酚和百里香酚含量的定量方法。
方法
样品用95%乙醇提取，采用ODS柱，以甲醇 - 水 - 乙酸（60:40:2）为流动相。检测波长设定为274 nm。
结果
香芹酚和百里香酚的线性范围分别为0.23 - 2.15微克（r = 0.9999）和0.39 - 2.36微克（r = 0.9999）；平均回收率分别为99.9%（RSD 1.4%）和98.6%（RSD 1.3%）；重复性RSD分别为1.1%和1.6%。
结论
该方法可靠，可用于石香薷的质量控制。
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