Mora S, Diaz-Veliz G, Lungenstrass H, García-González M, Coto-Morales T, Poletti C, De Lima T C M, Herrera-Ruiz M, Tortoriello J
Laboratorio de Farmacología del Comportamiento, Programa de Farmacología Molecular y Clínica, Facultad de Medicina, Universidad de Chile, P.O. Box 16038, Santiago, Chile.
J Ethnopharmacol. 2005 Feb 28;97(2):191-7. doi: 10.1016/j.jep.2004.10.028. Epub 2004 Dec 25.
In order to evaluate the effects produced by the hydroalcoholic extract of leaves from Casimiroa edulis on the central nervous system, different behavioral tests and animal models of depression and anxiety were performed. The extract was administered intraperitoneally in male and female rats and tested on spontaneous motor activity, locomotor activity, exploration of an elevated plus-maze (EPM) and in the forced swimming test (FST). In addition, the extract was administered orally in male and female mice and evaluated in the following tests: general observation, pentobarbital-induced hypnosis, EPM, rota-rod, hole-board, and marble-burying. The results revealed that, in rats, the extract caused considerable reduction of locomotor and exploratory activities and increased the exploration of the EPM open arms in a similar way that diazepam. In the FST, the extract was as effective as fluoxetine in inducing shortening of immobility, along with a significant increase on climbing duration. On the other hand, in mice, the extract prolonged pentobarbital-induced hypnosis, increased exploration of the EPM open arms and partially protected from the pentylenetetrazol-induced convulsions. No significant effect was evident on motor coordination, hole-board and marble-burying tests. These results suggest that the hydroalcoholic extract of Casimiroa edulis may contain sedative principles with potential anxiolytic and antidepressant properties, which need further investigation.
为了评估甜香瓜叶水醇提取物对中枢神经系统产生的影响，进行了不同的行为测试以及抑郁和焦虑动物模型实验。该提取物通过腹腔注射给予雄性和雌性大鼠，并对其自发运动活动、运动活性、高架十字迷宫（EPM）探索和强迫游泳试验（FST）进行测试。此外，该提取物通过口服给予雄性和雌性小鼠，并在以下测试中进行评估：一般观察、戊巴比妥诱导的催眠、EPM、转棒试验、洞板试验和埋大理石试验。结果显示，在大鼠中，该提取物导致运动和探索活动显著减少，并以与地西泮类似的方式增加了EPM开放臂的探索。在FST中，该提取物在诱导不动时间缩短方面与氟西汀一样有效，同时攀爬持续时间显著增加。另一方面，在小鼠中，该提取物延长了戊巴比妥诱导的催眠时间，增加了EPM开放臂的探索，并部分保护小鼠免受戊四氮诱导的惊厥。在运动协调、洞板试验和埋大理石试验中未观察到显著影响。这些结果表明，甜香瓜叶水醇提取物可能含有具有潜在抗焦虑和抗抑郁特性的镇静成分，这需要进一步研究。
