硫喷妥钠可防止大鼠海马切片的缺氧去极化，但丙泊酚或异氟烷不能。

Anoxic depolarization of rat hippocampal slices is prevented by thiopental but not by propofol or isoflurane.

作者信息

Sasaki R, Hirota K, Roth S H, Yamazaki M

机构信息

Department of Anaesthesiology, Toyama Medical and Pharmaceutical University of Medicine, 2630 Sugitani, Toyama, 930-0194, Japan.

出版信息

Br J Anaesth. 2005 Apr;94(4):486-91. doi: 10.1093/bja/aei077. Epub 2005 Feb 11.

DOI:10.1093/bja/aei077

PMID:15708869

Abstract

BACKGROUND

There is strong evidence to suggest that anoxic depolarization (AD) is an important factor in hypoxia/ischaemia-induced neural damage. Treatments that prevent the occurrence of AD may be useful in providing neuronal protection against hypoxia. The current study was designed to determine whether general anaesthetics which have been suggested to 'induce prophylaxis' against hypoxia can attenuate the incidence of AD.

METHODS

The effects of anoxia (3 min) on evoked extracellularly recorded field potentials of CA1 neurons in rat hippocampal slices were assessed in the absence and presence of the i.v. general anaesthetics thiopental and propofol and the volatile anaesthetic isoflurane.

RESULTS

In the absence of anaesthetics, AD occurred in 81% of the preparations tested. Thiopental (2 x 10(-4) M) significantly reduced the incidence of AD (16%, P=0.0006). In comparison, propofol (2 x 10(-4) M) and isoflurane (1.5 vol%) were ineffective (69% and 60%, respectively). Furthermore, in the presence of thiopental, the population spike amplitude recovered with and without AD (90% and 94% of pre-anoxic value, respectively) following 3 min anoxia.

CONCLUSION

The prophylactic effect of thiopental against hypoxia might be induced, in part, by preventing the generation of AD.

摘要

背景

有强有力的证据表明，缺氧去极化（AD）是缺氧/缺血诱导神经损伤的一个重要因素。预防AD发生的治疗方法可能有助于为神经元提供抗缺氧保护。本研究旨在确定已被认为对缺氧具有“诱导性预防作用”的全身麻醉剂是否能降低AD的发生率。

方法

在不存在和存在静脉注射全身麻醉剂硫喷妥钠和丙泊酚以及挥发性麻醉剂异氟烷的情况下，评估缺氧（3分钟）对大鼠海马切片中CA1神经元诱发的细胞外记录场电位的影响。

结果

在无麻醉剂的情况下，81%的受试制剂发生了AD。硫喷妥钠（2×10⁻⁴ M）显著降低了AD的发生率（16%，P = 0.0006）。相比之下，丙泊酚（2×10⁻⁴ M）和异氟烷（1.5体积%）无效（分别为69%和60%）。此外，在硫喷妥钠存在的情况下，缺氧3分钟后，无论是否发生AD，群体峰电位幅度均恢复（分别为缺氧前值的90%和94%）。