研究麻醉剂对大鼠海马切片缺氧损伤影响的方法。

Methods for studying the effect of anesthetics on anoxic damage in the rat hippocampal slice.

作者信息

Kass I S, Bendo A A, Abramowicz A E, Cottrell J E

机构信息

Anesthesiology Department, State University of New York Health Science Center, Brooklyn 11203.

出版信息

J Neurosci Methods. 1989 May;28(1-2):77-82. doi: 10.1016/0165-0270(89)90012-5.

DOI:10.1016/0165-0270(89)90012-5

PMID:2725017

Abstract

The rat hippocampal slice was used as a model system to study the effects of anesthetics on anoxic damage. Thiopental, but not isoflurane, allowed recovery of the postsynaptic population spike evoked from the dentate granule cells. Creatine preincubation protected both dentate granule and CA1 pyramidal cells against anoxic damage. Calcium-free, 10 mM magnesium artificial cerebrospinal fluid (ACSF) was shown to protect CA1 pyramidal cells against anoxic damage. The presynaptic population spike recovered to its preanoxic amplitude after anoxia even under conditions where the postsynaptic population spike demonstrated no recovery. Thus the hippocampal slice is a useful system for studying the effects of anesthetics and other pharmacological agents on anoxic damage.

摘要

大鼠海马切片被用作模型系统来研究麻醉剂对缺氧损伤的影响。硫喷妥钠可使齿状颗粒细胞诱发的突触后群体峰电位恢复，但异氟烷则不能。预先孵育肌酸可保护齿状颗粒细胞和CA1锥体细胞免受缺氧损伤。无钙、含10 mM镁的人工脑脊液（ACSF）可保护CA1锥体细胞免受缺氧损伤。即使在突触后群体峰电位未恢复的情况下，缺氧后突触前群体峰电位也恢复到缺氧前的幅度。因此，海马切片是研究麻醉剂和其他药理试剂对缺氧损伤影响的有用系统。