Ji Lanju, Ding Chenxu, Chen Guichen, Dai Donghai, Yang Yanrong
Northwest Plateau Institute of Biology, The Chinese Academy of Sciences, Xining 810001, China.
Se Pu. 2004 Jan;22(1):38-40.
A simple and accurate method for the determination of haleniaside and demethoxyhaleniaside in Halenia ellipitica D. Don. materials and patent medicines with high performance liquid chromatography (HPLC) was developed. The two components were extracted from powdered samples with methanol. The resultant extract was separated within 45 min on a VP-ODS C18 column (4.6 mm i.d. x 250 mm, 5 microm) under gradient elution with a mixture of acetonitrile-phosphate buffer at a flow rate of 1.0 mL/min. The detection wavelength was 254 nm and the injection volume was 20 microL. Under gradient elution program the volume fractions of acetonitrile in mobile phase were as follows: 0 - 5 min, 12%; 5 - 15 min, 12% - 15% ; 15 - 40 min, 15% - 35%. Both haleniaside and dehydrohaleniaside have good linearity in the ranges of 0.68 - 3.40 g/L and 0.36 - 1.8 g/L with correlation coefficients of 0.999 8 and 0.999 0 respectively. This method has been successfully applied to the analysis of Halenia ellipitica D. Don materials and related patent medicines.
建立了一种采用高效液相色谱法（HPLC）测定椭圆叶花锚药材及中成药中藏药苦苷和去甲氧基藏药苦苷的简单准确的方法。用甲醇从粉末状样品中提取这两种成分。所得提取物在VP-ODS C18柱（内径4.6 mm×250 mm，5μm）上，以乙腈-磷酸盐缓冲液混合物为流动相，在梯度洗脱条件下，于45分钟内实现分离，流速为1.0 mL/min。检测波长为254 nm，进样量为20μL。在梯度洗脱程序下，流动相中乙腈的体积分数如下：0 - 5分钟，12%；5 - 15分钟，12% - 15%；15 - 40分钟，15% - 35%。藏药苦苷和脱氢藏药苦苷在0.68 - 3.40 g/L和0.36 - 1.8 g/L范围内均具有良好的线性关系，相关系数分别为0.999 8和0.999 0。该方法已成功应用于椭圆叶花锚药材及相关中成药的分析。
