[Determination of haleniaside and demethoxyhaleniaside in wild and cultivated Halenia ellipitica D. Don. by reversed-phase high performance liquid chromatography].

A simple and accurate method for the determination of haleniaside and demethoxyhaleniaside in Halenia ellipitica D. Don. materials and patent medicines with high performance liquid chromatography (HPLC) was developed. The two components were extracted from powdered samples with methanol. The resultant extract was separated within 45 min on a VP-ODS C18 column (4.6 mm i.d. x 250 mm, 5 microm) under gradient elution with a mixture of acetonitrile-phosphate buffer at a flow rate of 1.0 mL/min. The detection wavelength was 254 nm and the injection volume was 20 microL. Under gradient elution program the volume fractions of acetonitrile in mobile phase were as follows: 0 - 5 min, 12%; 5 - 15 min, 12% - 15% ; 15 - 40 min, 15% - 35%. Both haleniaside and dehydrohaleniaside have good linearity in the ranges of 0.68 - 3.40 g/L and 0.36 - 1.8 g/L with correlation coefficients of 0.999 8 and 0.999 0 respectively. This method has been successfully applied to the analysis of Halenia ellipitica D. Don materials and related patent medicines.