Song Yali, Hu Fengzu, Lin Pengcheng, Lu Yongchang, Shi Zhixian
Northwest Plateau Institute of Biology, The Chinese Academy of Sciences, Xining 810001, China.
Se Pu. 2004 Jan;22(1):51-3.
A simple and rapid method for simultaneous separation and determination of xanthones in Swertia mussotii and Swertia franchetiana by high performance liquid chromatography has been established. The analysis was performed on a Kromasil C18 column (250 mm x 4.60 mm i.d., 5 microm) (at 20 degrees C) eluted with methanol and 0.1% aqueous phosphoric acid (73: 27 in volume ratio) as mobile phase at a flow rate of 1.0 mL/min and with UV detection at 260 nm. Results showed that xanthones were separated successfully from each other and from other interfering components. There was a good linear relationship between the content of component and its peak area for each xanthone, with the correlation coefficients of 0.999 2 - 0.999 9. The convenient method can be used for quantitative analysis of xanthones.
建立了一种采用高效液相色谱法同时分离和测定川西獐牙菜和川东獐牙菜中呫吨酮类成分的简单快速方法。分析在Kromasil C18柱（250 mm×4.60 mm内径，5μm）上进行（20℃），以甲醇和0.1%磷酸水溶液（体积比73:27）作为流动相，流速为1.0 mL/min，在260 nm处进行紫外检测。结果表明，呫吨酮类成分彼此之间以及与其他干扰成分成功分离。各呫吨酮类成分的含量与其峰面积之间具有良好的线性关系，相关系数为0.999 2 - 0.999 9。该简便方法可用于呫吨酮类成分的定量分析。
