Nagata Toshifumi, Yamada Hitomi, Du Zeji, Todoriki Setsuko, Kikuchi Shoshi
National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
J Agric Food Chem. 2005 Feb 23;53(4):1022-30. doi: 10.1021/jf0486895.
To reveal the signal transduction mechanism of the response to stress in the form of active oxygen species, we used a microarray system to analyze gene expression patterns 2 or 24 h after gamma-irradiation of Arabidopsis. gamma-Irradiation induces several signal transduction and metabolite genes. By analysis of cis-elements located on the promoter region of the gamma-responsive genes, we have also found several cis-elements related to various signal transduction systems. We also analyzed the pleiotropic mutant ttg1-1, which has a dramatically altered physiological response to gamma-irradiation. By comparing the gene expression patterns of wild-type (Ler) and ttg1-1 mutant plants after gamma-irradiation, we identified various TTG1-regulated gamma-response genes. Analysis of the cis-elements in the promoter region of the gamma-responsive genes also revealed that the many transcription factors interacting with TTG1 protein (WD40 protein) are related to the gamma-responsive gene expression.
为了揭示植物对活性氧形式的应激反应的信号转导机制，我们使用微阵列系统分析了拟南芥经γ射线照射2小时或24小时后的基因表达模式。γ射线照射诱导了几个信号转导和代谢物基因。通过分析γ反应基因启动子区域的顺式元件，我们还发现了几个与各种信号转导系统相关的顺式元件。我们还分析了多效性突变体ttg1-1，其对γ射线照射的生理反应发生了显著改变。通过比较γ射线照射后野生型（Ler）和ttg1-1突变体植株的基因表达模式，我们鉴定出了各种受TTG1调控的γ反应基因。对γ反应基因启动子区域顺式元件的分析还表明，许多与TTG1蛋白（WD40蛋白）相互作用的转录因子与γ反应基因表达有关。
