Glucosinolate O-methyltransferase mediated callus formation and affected ROS homeostasis in .

UNLABELLED

Auxin-induced callus formation was largely dependent on the function of Lateral Organ Boundaries Domain (LBD) family transcription factors. We previously revealed that two () genes, and , may be involved in the callus formation process as potential target genes of . Overexpression of the genes induces spontaneous callus formation. However, the details of the involvement in callus formation process were not well studied. , but not , are targeted and induced by LBD29 during the early stage of callus formation. Cell membrane and nucleus localized IGMT3 was mainly expressed in the elongation and maturation zones tissues of the primary root and lateral root, which could be further accumulated after CIM treatment. The quadruple mutant, which obtained by CRISPR/Cas9 technology, exhibits a phenotype of attenuated callus formation. Enhanced indole glucosinolate anabolic pathway caused by overexpression promotes callus formation. In addition, the genes were involved in the reactive oxygen species homeostasis, which could be responsible for its role on callus formation. This study provides novel insights into the role of gene-mediated callus formation. Activation of the Indole glucosinolate anabolic pathway is an inducing factor for plant callus initiation.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s12298-023-01409-2.