Dufrane D, Goebbels R M, Fdilat I, Guiot Y, Gianello P
Laboratory of Experimental Surgery, Faculty of Medicine, Université catholique de Louvain, Brussels, Belgium.
Pancreas. 2005 Mar;30(2):138-47. doi: 10.1097/01.mpa.0000147083.62501.4e.
To study the impact of porcine islet size on structural properties and cellular engraftment.
The endocrine structure and collagen/vascular localization in pig islets were studied before and after enzymatic isolation on the pancreas from 6 young and 6 adult Landrace pigs. Isolated islets from both pig types were transplanted under the kidney capsula of diabetic nude rats to assess cellular engraftment.
In comparison with adult pig pancreata, a significantly greater number of small beta cells (<100 microm) were observed before and after isolation (82% vs. 32%, respectively, P < 0.005) from young pig pancreata. Small islets (<100 microm) showed a peripheral vascular structure, whereas large islets showed a more centralized vascular organization, thereby providing protection during the enzymatic digestion procedure. The islet endocrine structure was not affected by the islet size, but a loss of glucagon cells (-7.9%, P < 0.005) was observed in large isolated islets. The purity of islet preparation was better with pancreata from adult than young donors (86% vs. 64%, respectively, P < 0.05). A lack of engraftment was observed for small islets from young pig donors as compared with large islets from adult donors.
Large and well-structured islets, mainly found in adult pig pancreata, probably possess a better potential for cellular engraftment due to centralized vascularization and collagen distribution.
研究猪胰岛大小对其结构特性和细胞植入的影响。
对6只幼年和6只成年长白猪胰腺进行酶分离，研究分离前后猪胰岛的内分泌结构以及胶原/血管定位。将两种猪的分离胰岛移植到糖尿病裸鼠肾被膜下，以评估细胞植入情况。
与成年猪胰腺相比，幼年猪胰腺分离前后观察到的小β细胞（<100微米）数量明显更多（分别为82%和32%，P<0.005）。小胰岛（<100微米）呈现外周血管结构，而大胰岛呈现更集中的血管组织，从而在酶消化过程中提供保护。胰岛内分泌结构不受胰岛大小影响，但在大的分离胰岛中观察到胰高血糖素细胞减少（-7.9%，P<0.005）。成年供体胰腺制备的胰岛纯度优于幼年供体（分别为86%和64%，P<0.05）。与成年供体的大胰岛相比，幼年猪供体的小胰岛缺乏植入。
主要在成年猪胰腺中发现的大且结构良好的胰岛，可能由于集中的血管化和胶原分布而具有更好的细胞植入潜力。
